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Cdk5-HA

Plasmid

#1872

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk5 (CDK5 Human)

Tags

HA

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_WT

Plasmid

#81795

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

Plasmid

#138461

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-VP64-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBP-Bba_K822000

Plasmid

#74094

Purpose

L-Lactate inducible promoter from E. coli MG1655

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_K822000 (L-Lactate promoter - E. coli MG1655)

Use

Synthetic Biology

Expression

Bacterial

Promoter

NA

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304-Flag-APEX2-NES

Plasmid

#92158

Purpose

Cytosolic APEX2 construct

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

Flag-APEX2-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-cj-E sgRNA

Plasmid

#169915

Purpose

Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 CMV sTurboID (C)-Arrestin-GFP

Plasmid

#153010

Purpose

Expresses split-TurboID C-terminal fragment fused to arrestin

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (C)(G74)-Arrestin

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDR121

Plasmid

#37148

Depositor

Dale Ramsden

Insert

XRCC6 (XRCC6 Human)

Tags

H6

Expression

Insect

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
pTO149_RFP_CauNEO

Plasmid

#177277

Purpose

Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

RFP, CauNEO

Tags

ENO1-RFP

Expression

Bacterial

Mutation

All CUG Leucine codons were replaced with the syn…

Promoter

AgTEF2

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR10-YFP

Plasmid

#13643

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 10 (TLR10 Human)

Tags

YFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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