We narrowed to 4,345 results for: Ado;

1. pLKO-Tet-On-YAP1-shRNA2

   Plasmid

   #176847

   Purpose

   lentiviral vector for doxycycline-inducible human YAP1 knockdown

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   YAP1-Sh2 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28 His-MBP-TEV-BAP-Human BCKDK

   Plasmid

   #232116

   Purpose

   For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   BCKDK (BCKDK Human)
   

   Use

   Tags

   6 x His, Biotin acceptor peptide (BAP), Maltose-B…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

   Plasmid

   #171802

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-mgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

   Plasmid

   #171630

   Purpose

   Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

   Depositor

   Christopher A French, Chris French

   Article

   Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   BioTAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

   Plasmid

   #87109

   Purpose

   BPNLS-Cas9-BPNLS-2AGFP expression plasmid

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   1BPNLS-Cas9-1BPNLS-2A-EGFP
   

   Use

   CRISPR

   Tags

   3xFLAG (on Cas9)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-EF1a ANXA11-mEmerald D40G

   Plasmid

   #164211

   Purpose

   pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   D40G

   Promoter

   EF1a

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-CRY2oligo-mcherry-ANXA11(D40G)

   Plasmid

   #164204

   Purpose

   Expresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Tags

   CRY2oligo-mcherry

   Expression

   Mammalian

   Mutation

   D40G

   Promoter

   CMV

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-CRY2oligo-mCherry-ANXA11(186-505 aa)

   Plasmid

   #164208

   Purpose

   Expresses CRY2oligo-mcherry tagged ANXA11 C terminal under CMV promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Tags

   CRY2oligo-mcherry

   Expression

   Mammalian

   Mutation

   186-505 aa

   Promoter

   CMV

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-CRY2oligo-mcherry-ANXA11(1-185aa)

   Plasmid

   #164207

   Purpose

   Expresses CRY2oligo-mcherry tagged ANXA11 N terminal under CMV promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Tags

   CRY2oligo-mcherry

   Expression

   Mammalian

   Mutation

   1-185 aa

   Promoter

   CMV

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPHR-mCherry-mHP1alpha

    Plasmid

    #181883

    Purpose

    Soluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. JDW 742 (pDONR221-EGFP-KRAS4B-G12V)

    Plasmid

    #156403

    Purpose

    Gateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4B-G12V (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    EGFP

    Expression

    Mutation

    mutant KRAS4B-G12V

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPHR-mCherry-hNPM

    Plasmid

    #181887

    Purpose

    Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hNPM (NPM1 Mustard Weed, Human)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPHR-mCherry-hHP1gamma

    Plasmid

    #181885

    Purpose

    Soluble light-dependent effector protein (human HP1gamma) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hHP1gamma (CBX3 Mustard Weed, Human)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR D-TOPO-C3_33

    Plasmid

    #60275

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    GLIS3 enhancer (GLIS3 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)

    Plasmid

    #156410

    Purpose

    pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12V

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12V (KRAS Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12V

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)

    Plasmid

    #156409

    Purpose

    pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12D

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.23 C3_13

    Plasmid

    #60298

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    EDN3 enhancer (EDN3 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. CMV-CRY2oligo-mcherry-ANXA11(R235Q)

    Plasmid

    #164205

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 R235R mutant under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    R235Q

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)

    Plasmid

    #156407

    Purpose

    pCS2 CMV expression vector driving EGFP fused human KRAS4B-G12V

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4B-G12V (KRAS Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    mutant KRAS4B-G12V

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPHR-mCherry-hHP1alpha

    Plasmid

    #181884

    Purpose

    Soluble light-dependent effector protein (human HP1alpha) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hHP1alpha (CBX5 Mustard Weed, Human)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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