We narrowed to 2,443 results for: CLU

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pET19-M(BRCT)TdTwt

Plasmid

#207459

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

M(BRCT)TdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMVmin-BC0524-luc2

Plasmid

#227139

Purpose

Barcoded assay, Ca2+ sensor; barcode BC0524

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

6x clustered NFAT element linked to CMV minimal promoter driving barcode 0524 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-recA56

Plasmid

#89962

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet and proD

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-3x-p67phox-GBD

Plasmid

#214141

Purpose

Rac activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xp67phox-GBD (NCF2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti 4.1 Ex miR200c-141

Plasmid

#35534

Purpose

3rd generation lentiviral vector

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

miR200c-141 genomic cluster (MIR200C, MIR141 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapErk)dTomato

Plasmid

#200113

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

MapErk sequence in minimal promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

5 copies of designed MapErk sequence, no other pr…

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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MYR-FR-C2D-EGFR

Plasmid

#179262

Purpose

Light-induced clustering of EGFR cytosolic domains

Depositor

Jared Toettcher

Article

Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Cry2 PHR, FUS, FusionRed, and Myristoylation sequ…

Promoter

SFFV

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pKrox24(2xD-E)dTomato

Plasmid

#200110

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

modified EGR1 promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

2 copies of D-E element, no other promoter elemen…

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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FGFR1-optoDroplet

Plasmid

#111509

Purpose

For optogenetic clustering of FGFR1

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

FGFR1 (FGFR1 Human)

Use

Lentiviral

Tags

Cry2, FUS, Fusion Red, and Myristoylation sequenc…

Promoter

SFFV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSBtet rtTA G72V SE Δsplice GP Luc

Plasmid

#194328

Purpose

Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness

Depositor

Alexander McLellan

Article

Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:

Insert

Luciferase

Use

Luciferase

Expression

Mammalian

Mutation

Tet-responsive promotor related mutations: G72V i…

Promoter

Tight tet-responsive promotor

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_linker

Plasmid

#211731

Purpose

Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Linker fragment SARS-CoV-2

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0396-luc2

Plasmid

#227115

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0396

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0396 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_CRE-CMVmin-BC0491-luc2

Plasmid

#227133

Purpose

Barcoded assay, cAMP/Ca2+ sensor; barcode BC0491

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

6x clustered CRE element linked to CMV minimal promoter driving barcode 0491 and a luciferase reporter gene

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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p-spTorA-GFP-H6C

Plasmid

#168517

Purpose

GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

spTorA-GFP-H6C

Tags

6xHisC tag and spTorA (signal peptide of E. coli …

Expression

Bacterial

Mutation

The mut3 GFP variant with 5 additional mutations …

Promoter

araBAD

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMVmin-BC0538-luc2

Plasmid

#227140

Purpose

Barcoded assay, Ca2+ sensor; barcode BC05038

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p55-H2B-Dendra2

Plasmid

#80609

Purpose

Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

H2B-Dendra2 (H2BC21 Human, Synthetic)

Tags

Dendra2

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

Plasmid

#190153

Purpose

Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp2 (Vamp2 Mouse)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only AA #1-94

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCD4-TagRFP-T (RHPMP151)

Plasmid

#119238

Purpose

Expresses CD4 fused to TagRFP-T

Depositor

Ricardo Henriques

Article

Pereira et al Front Immunol. 2019 Apr 5;10:675. doi: 1

Insert

CD4 (CD4 Human)

Tags

tagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCsy_complex

Plasmid

#89232

Purpose

Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Inserts

Csy1
Csy2
Csy3
Csy4

Tags

6xHis-TEV protease

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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