We narrowed to 41,264 results for: Tro

1. pLentiCRISPRv1_SDHB

   Plasmid

   #177981

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHB (SDHB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-neo-TET

   Plasmid

   #51286

   Purpose

   expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   U6 (RNU6-1 Human)
   

   Use

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Mutation

   Promoter

   U6 upstream enhancer sequence

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv1_SDHA

   Plasmid

   #177980

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHA (SDHA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SspB-HaloTag-DHPH-TIAM1

   Plasmid

   #176114

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SspB-mCherry-DHPH-TIAM1

   Plasmid

   #176104

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBactin-Halo-Tag

   Plasmid

   #196898

   Purpose

   Expression of Halo-Tag. Use as control for Halo-tag fusions

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Halo-Tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. SspB-iRFP-DHPH-TIAM1

   Plasmid

   #176118

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE-SSeCKs T751A

   Plasmid

   #107142

   Purpose

   full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

   Depositor

   Heidi Hehnly

   Article

   Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

   Insert

   SSeCKs T751A (Akap12 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed threonine 751 to alanine

   Promoter

   gag

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-ΔN-RNMT

   Plasmid

   #112708

   Purpose

   Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNMT (RNMT Human)
   

   Use

   Tags

   FLAG and HIV Rev nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   deleted amino acids 2-120, mutated active-site D2…

   Promoter

   CMV

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SHIP2 WT pBABE-His-Xpress

    Plasmid

    #126584

    Purpose

    Retroviral infection of MEF cells (puromycin selection)

    Depositor

    Christophe Erneux

    Article

    Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

    Insert

    SHIP2 (INPPL1 Human)
    

    Use

    Retroviral

    Tags

    His Xpress (N-terminal)

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIBN-TetR

    Plasmid

    #103813

    Purpose

    BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-TetR (CIB1, tetR E. coli, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-hs-3xHA-delta6xNHL-LIN-41

    Plasmid

    #52722

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 593–868

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-gfap-sgRNA

    Plasmid

    #65566

    Purpose

    in vitro trancription of sgRNA targeting the zebrafish gfap locus

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    gfap sgRNA (gfap Zebrafish)
    

    Use

    In vitro rna transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-mCherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176138

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MO70-hJunctate

    Plasmid

    #79596

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

    Insert

    junctate (ASPH Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pMXs-hs-3xHA-deltaRING-LIN-41

    Plasmid

    #52718

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 12-91

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

    Plasmid

    #69899

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (stw Fly, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV[Tet]-Puro-TRE3G>mMyod1[NM_010866.2]

    Plasmid

    #184380

    Purpose

    The mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G promoter

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Myod1 (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176135

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MO70-hJunctate - EF-mutant

    Plasmid

    #79597

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

    Insert

    junctate (ASPH Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    77DAD79 to 77AAA79

    Promoter

    CMV

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only