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hRad51(F86E)–Cas9(D10A)

Plasmid

#125564

Purpose

Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(F86E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFP122

Plasmid

#21669

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 A. victoria, Budding Yeast)

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Available Since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

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FLAG-mPar6a WT

Plasmid

#86148

Purpose

FLAG tagged mouse Par6 wild type - Binds to aPKC, Par3, Lgl, Cdc42

Depositor

Senthil Muthuswamy

Article

Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

Insert

Par6 (Pard6a Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

LTR

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only
pAc5.1A-Dm-miR-13b-1

Plasmid

#22762

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 13b-1 (mir-13b-1 primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pRY1001

Plasmid

#21787

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

CFP-Ste12 (STE12 A. victoria, Budding Yeast)

Tags

CFP

Expression

Yeast

Mutation

contains 1000 bp UTR (Ste12 promoter) upstream of…

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO.1-puro-shGFP

Plasmid

#247239

Purpose

shRNA control targeting GFP

Depositor

Miguel Ramalho-Santos

Article

Cho et al Oncogene. 2026 Apr;45(13):1198-1210. doi

Insert

negtive control shRNA targeting GFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pSHARE-TRACE-mU1-empty

Plasmid

#253332

Purpose

Lineage tracing vector compatible with SHARE-seq, modified from Weinreb at al 2020. Expresses GFP from EF1a promoter with a mouse U1 inserted into 3' UTR to improve nuclear localization.

Depositor

Jason Buenrostro

Article

Nagaraja et al Nature. 2026 Mar 25. doi: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamM(pre-cut)AB

Plasmid

#248942

Purpose

Hachiman from P. fluorescens under pTet control with HamM programmed breaks in the peptide backbone at I118 and I218

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamM(pre-cut)AB

Expression

Bacterial

Mutation

HamM programmed breaks in the peptide backbone at…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pSBbi-MTS-FAP-GFP

Plasmid

#242990

Purpose

Expresses MTS-FAP-GFP in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

MTS-dH6.2-GFP

Use

Sleeping beauty

Tags

GFP

Expression

Mammalian

Promoter

EF-1⍺

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBbi-FAP-GFP-KDEL

Plasmid

#242991

Purpose

Expresses FAP-GFP-KDEL in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

dH6.2-GFP-KDEL

Use

Sleeping beauty

Tags

GFP

Expression

Mammalian

Promoter

EF-1⍺

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 Hygro sgIKBKG_1

Plasmid

#241449

Purpose

Delete IKBKG

Depositor

Richard Possemato

Insert

sgIKBKG_1 (IKBKG Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 Hygro sgIKBKG_2

Plasmid

#241450

Purpose

Delete IKBKG

Depositor

Richard Possemato

Insert

sgIKBKG_2 (IKBKG Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEPHS1CB0027

Plasmid

#227570

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_TkTXSS (T374G/E379D/Q486H/A745P)_35S

Expression

Plant

Mutation

T374G / E379D / Q486H / A745P, BsaI sites removed…

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN12-Clover

Plasmid

#215527

Purpose

Expression vector with clover-tagged PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Tags

Clover

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINDUCER20-PTPN12

Plasmid

#215517

Purpose

Dox-inducible lentiviral transfer vector with PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Lentiviral

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN12-Clover

Plasmid

#215521

Purpose

Gateway entry vector with clover-tagged PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway Cloning

Tags

Clover

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(E378G)

Plasmid

#215462

Purpose

Gateway entry vector with Src constitutively-active mutant (E378G)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway Cloning

Mutation

E378G (constitutively-active)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-VPS35-mScarlet

Plasmid

#215472

Purpose

Gateway entry vector with VPS35 tagged with mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

VPS35 (VPS35 Human)

Use

Gateway Cloning

Tags

mScarlet

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(Y527F)

Plasmid

#215460

Purpose

Gateway entry vector with Src constitutively-active mutant (Y527F)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway Cloning

Mutation

Y527F (constitutively-active)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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