We narrowed to 22,775 results for: crispr

1. pB[U6-3 wex2 PUb-DsRed]

   Plasmid

   #169010

   Purpose

   For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   white ex2 sgRNA
   

   Use

   Piggybac

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila melanogaster U6:3

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK2101

   Plasmid

   #65770

   Purpose

   Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized SaCas9, and SaCas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS-3xFlag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. spCas9-NLS-MAV

   Plasmid

   #124212

   Purpose

   SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

   Depositor

   Uri David Akavia

   Article

   Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   His6, MBP, TEV Cleavage site, and two copies of S…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. BB3cN_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104906

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC2-dual-dCas9VP48-sgExpression

   Plasmid

   #48236

   Purpose

   Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9VP48
   

   Use

   CRISPR

   Tags

   HA-tag and VP48

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-NLS-AcrIIA4

   Plasmid

   #113038

   Purpose

   AAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SV40 NLS-AcrIIA4
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

   Plasmid

   #102887

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

   Plasmid

   #120297

   Purpose

   AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC5-dual-dCas9VP48-sgTetO

    Plasmid

    #48237

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48 and sgTetO
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102891

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS540-pCSDest2-AcrIIC4Hpa

    Plasmid

    #113434

    Purpose

    Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS542-pCSDest2-AcrIIC5Smu

    Plasmid

    #113435

    Purpose

    Expresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSC51

    Plasmid

    #104825

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.12g075700, Glyma.11g145900
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

    Plasmid

    #120300

    Purpose

    AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV CMV-driven AcrIIC1-2xmiR-122 target sites

    Plasmid

    #120302

    Purpose

    AAV vector for expression of AcrIIC1 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC1-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV CMV-driven AcrIIC3-2xmiR-122 target sites

    Plasmid

    #120303

    Purpose

    AAV vector for expression of AcrIIC3 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC3-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pJJB308

    Plasmid

    #107699

    Purpose

    AtU6 Promoter-LwaCas13a-gRNA Scaffold

    Depositor

    Daniel Voytas

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330S-2-PITCh

    Plasmid

    #63670

    Purpose

    Expresses Cas9 nuclease and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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