We narrowed to 10,599 results for: cat.1
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Plasmid#211963PurposeExpress gRNA against FOXH1 with puro and BFPDepositorInsertsgRNA targeting FOXH1 (FOXH1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I no force control (F40-based)
Plasmid#118726PurposeThe no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.DepositorInserthuman Desmoplakin I (1-1952)-[YPet(short)-F40-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationtruncation after aa1952PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBE44
Plasmid#174654PurposeCAPTURE method 15A origin of replication receiver plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBE48
Plasmid#174656PurposeCAPTURE method BAC origin of replication receiver plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLB
Plasmid#11619PurposepLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsGFPExpressionMammalianMutationPromotermouse U6Available sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRibo EcoHind
Plasmid#36252DepositorTypeEmpty backboneUseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModified Hsp60Available sinceApril 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_OriM
Plasmid#110097PurposeAnalogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3H
Plasmid#110080PurposeAnalogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pREPK219H
Plasmid#51492PurposepREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.DepositorInsertRep/RepA
UsePlant expression t-dnaTagsExpressionMutationchanged lysine 219 to histidinePromoter2x35SAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMEXNH3
Plasmid#110079PurposeAnalogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTagsHis10 Tag - 3C cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-PheB
Plasmid#71738PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertPheB
UseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterpRplsWTAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0281
Plasmid#162302PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, [S-tag]-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRiboS BsaHind
Plasmid#36253DepositorTypeEmpty backboneUseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModified Hsp60Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRibo BsaHind
Plasmid#36251DepositorTypeEmpty backboneUseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModified Hsp60Available sinceMay 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
QUAS-5-T7-GFP_ColE1
Plasmid#171653PurposeQUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-5-T7
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0550
Plasmid#162301PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-SUMO-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0244
Plasmid#162282PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPMY0SP0002
Plasmid#162304PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, 6xHis tag-[HRV 3C cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0551
Plasmid#162303PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0547
Plasmid#162298PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, TrxA-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0546
Plasmid#162297PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0544
Plasmid#162295PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[Factor Xa cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0543
Plasmid#162294PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, GST-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0541
Plasmid#162292PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, GST-[thrombin cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
UseTagsExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Kv-ArcLight-ST
Plasmid#182319PurposeSoma-targeted fluorescent voltage indicator with left-shifted voltage-dependencyDepositorInsertKv-ArcLight-ST
UseTagsExpressionMammalianMutationMutation in an amino acid Q217E of ArcLight-MTPromoterCAGAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBLego
Plasmid#219967PurposeThe pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.DepositorTypeEmpty backboneUseTagsSLEHHHHHH and pelB signal sequenceExpressionBacterialMutationPromoterpBADAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
PRDX6A-c001
Plasmid#175474PurposeProtein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.DepositorInsertPRDX6 (PRDX6 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationSee Depositor CommentsPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6.3>gRNA.f+e
Plasmid#99139PurposeEmpty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoterDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterchicken U6.3Available sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEX128·gRNA
Plasmid#187600PurposeTemplate for amplification of gRNA with Cas6 recognition siteDepositorInsertgRNA template
UseTagsExpressionMutationPromoterno promoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-TARG1
Plasmid#172594PurposeFor purification (from E.coli) of His-tagged TARG1DepositorInsertTARG1 (OARD1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pOGG004
Plasmid#113979PurposeVector construction module. Level 1 golden gate cloning site module with LacZ reporterDepositorInsertLevel 1 golden gate cloning site
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-8xHis
Plasmid#101699Purpose8xHis in domestication vectorDepositorInsertAATG-[8xHis]-AGCC
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
a4 ecto pcDNA3.1 hygro(-)
Plasmid#113406Purposeexpression of human alpha 4 ectodomain with purification tags and C-terminal claspDepositorInserthuman alpha 4 ectodomain
UseTagsStrep II tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Halo XLF sgRNA
Plasmid#207605PurposesgRNA for the insert of the HaloTag at the endogenous loci of XLFDepositorInsertGTTCTTCCATctgcaaaaaa
UseTagsNoneExpressionMammalianMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
HaloKu70 sgRNA
Plasmid#207583PurposesgRNA for the insert of the HaloTag at the endogenous loci of Ku70.DepositorInsertGAGCAGTAGCCAACATGTCA
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTA2EGFP
Plasmid#193756PurposeExpresses EGFP under the control of PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPLTetO-1Available sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTC3RBSC1cI
Plasmid#193773PurposeExpresses lambda CI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertLambda CI
UseTagsExpressionBacterialMutationPromoterPLTetO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLC3RBSC2cI
Plasmid#193775PurposeExpresses lambda CI under the control of PLLacO-1 promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertLambda CI
UseTagsExpressionBacterialMutationPromoterPLLacO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only