We narrowed to 3,184 results for: actin

1. pET29b-clbI S178A

   Plasmid

   #114159

   Purpose

   Expresses ClbI S178A

   Depositor

   Emily Balskus

   Article

   Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

   Insert

   clbI
   

   Use

   Tags

   6xHis and 6xHis-Thrombin site

   Expression

   Bacterial

   Mutation

   Mutated S178 to Ala

   Promoter

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-clbH-C-A2-PCP

   Plasmid

   #114157

   Purpose

   Expresses ClbH-C-A2-PCP

   Depositor

   Emily Balskus

   Article

   Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

   Insert

   clbH
   

   Use

   Tags

   6xHis-thrombin-T7 tag

   Expression

   Bacterial

   Mutation

   Only contains the C-A2-PCP domains of ClbH

   Promoter

   Available since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas8

   Plasmid

   #82380

   Purpose

   dCas9 without a promoter in acsA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas73

   Plasmid

   #82398

   Purpose

   Δccm:Km, used to delete ccm operon

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   ccm deletion
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLG-deltaA

   Plasmid

   #33151

   Depositor

   Michael Rosbash

   Article

   Legrain et al Cell. 1989 May 19;57(4):573-83.

   Insert

   RP51A-synthetic minimal intron (RPS17A Budding Yeast)
   

   Use

   Tags

   LacZ

   Expression

   Yeast

   Mutation

   The RP51A sequence was modified by digestion with…

   Promoter

   Available since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

6. 413-Met25 Kar2 TorsinA E171Q HDEL

   Plasmid

   #32123

   Depositor

   Susan Lindquist

   Article

   Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

   Insert

   TorsinA (TOR1A Human)
   

   Use

   Tags

   HDEL and Kar2

   Expression

   Yeast

   Mutation

   K108A

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

7. 413-Met25 Kar2 TorsinA K108A HDEL

   Plasmid

   #32121

   Depositor

   Susan Lindquist

   Article

   Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

   Insert

   TorsinA (TOR1A Human)
   

   Use

   Tags

   HDEL and Kar2

   Expression

   Yeast

   Mutation

   K108A

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pL1P4 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198048

   Purpose

   MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pL1P3 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198047

   Purpose

   MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid

    #36047

    Purpose

    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA(R118G)-HA
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    Available since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HIR WT

    Plasmid

    #24049

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK5-mFzd8

    Plasmid

    #42260

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 8 (Fzd8 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pMP2463

    Plasmid

    #107774

    Purpose

    Expression of EGFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EGFP in pBBR1-mcs5
    

    Use

    Tags

    pLacZ-EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    pLacZ

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs-IP-EGFP-ULK1

    Plasmid

    #38193

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-p150

    Plasmid

    #51412

    Purpose

    expression of full length of chicken DCTN1 p150Glued in mammalian cells

    Depositor

    Trina Schroer

    Article

    Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

    Insert

    DCTN1 (DCTN1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pNVLTv2-pvdIJD-Prha-base

    Plasmid

    #169244

    Purpose

    Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    rhaRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaS

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKEK3228

    Plasmid

    #234090

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    aeBlue
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HIR K1030R

    Plasmid

    #24050

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K1030R

    Promoter

    Available since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-mFzd3

    Plasmid

    #42255

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 3 (Fzd3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only