We narrowed to 13,994 results for: eng
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Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
TagsAmilCP and RTXExpressionBacterialPromoterJ23100 promoterAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry
Plasmid#85434PurposeLentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expressionDepositorInsertPembrolizumab
UseLentiviralAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF1
Plasmid#155346PurposeExpress full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cellsDepositorAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ151-HDR
Plasmid#113631PurposeContains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.DepositorInsertsLeft homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
UseCre/Lox; Homology recombination selection vectorExpressionBacterial and MammalianPromoterEF1a promoter, EF1a promoter (T2A cleaving sequen…Available SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpG-P2A-GFP
Plasmid#159980PurposePrime editing in mammalian cellsDepositorInsertPE2-SpG-P2A-GFP
TagsBpSV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta13
Plasmid#13559DepositorInsertFLIPglu F16A Delta13
TagsECFP and EYFPExpressionBacterialMutationF16A in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pT7-eGFP
Plasmid#32552DepositorAvailable SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-MA4-GFP
Plasmid#114380PurposeDoxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpG
Plasmid#159978PurposePrime editing in mammalian cellsDepositorInsertPE2-SpG
TagsBpSV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x4
Plasmid#58768PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-WT(732)-8h
Plasmid#154101PurposeMammalian expression plasmid for dimeric soluble ACE2 (8his tag)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable SinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)
Plasmid#145147PurposeMammalian expression plasmid for soluble ACE2 (protease domain)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM491-Flag-His
Plasmid#109282PurposeORBIT integrating plasmid for C-terminal tagging with FLAG-His tagDepositorInsertattB-Flag-His
UseOrbit integrating plasmidAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-tTA
Plasmid#99119PurposeAn AAV genome that expresses tTA from the hSyn1 promoterDepositorInserttTA
UseAAVExpressionMammalianMutation*(see below)PromoterhSyn1Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1(TATV)(BB) (pY221)
Plasmid#89354PurposeExpresses humanized AsCpf1 TATV PAM variant and crRNA guideDepositorInsertCpf1 RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCBhAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP
Plasmid#31477DepositorInsertSFFV HA NLS Y2 T2A mTagBFP
UseLentiviralTagsHA and NLSMutationF13Y + S111Y on I-AniI = Y2 "reo" (res…Available SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-tdTomato
Plasmid#99116PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomatoDepositorInsertDIO-tdTomato
UseAAVExpressionMammalianPromoterTREAvailable SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSP64TS (DM#117)
Plasmid#15034DepositorInsertbeta-globin 5' and 3' UTR
ExpressionBacterialAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25F
Plasmid#91143PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
UseCRISPRExpressionPlantAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only