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pAAV-CWB-cyan pre-eGRASP(p30)

Plasmid

#111586

Purpose

An AAV vector that expresses cyan pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p30)

Use

AAV

Promoter

CaMKIIa

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Publication

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330-SpCas9-HF1

Plasmid

#108301

Purpose

pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pnsgRNA

Plasmid

#172717

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Publication

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

J23119-gRNA

Expression

Bacterial

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-3_synNotch_Gal4VP64

Plasmid

#85422

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-3 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her_4D5-3 synNotch

Use

Lentiviral

Promoter

PGK

Available since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAW005-2

Plasmid

#85611

Purpose

Empty gRNA delivery vector for Bacillus subtilis

Depositor

Perry Chou

Publication

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-flex-oChIEF-citrine

Plasmid

#50973

Purpose

flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

Depositor

Roger Tsien

Publication

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

inverted and inserted between two lox sites

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-GFAP-mKate2.5f

Plasmid

#99129

Purpose

Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mKate2.5f

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pZHY013

Plasmid

#36185

Depositor

Daniel Voytas

Publication

Zhang et al Plant Physiol. 2012 Nov 2.

Insert

Heterodimeric FokI nuclease

Use

Entry vector for expression in plants as a t2a li…

Promoter

none

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pG1K

Plasmid

#71741

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Publication

Reeve et al ACS Synth Biol. 2016 Jul 1.

Type

Empty backbone

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBR.DT-A

Plasmid

#35955

Depositor

Jacqueline Lees

Publication

Danielian et al Oncogene. 2008 Nov 20;27(51):6561-70. Ep

Insert

diphtheria toxin A

Use

Bac recombineering

Promoter

PGK

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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977 pSG5L HA PTEN A4

Plasmid

#10753

Depositor

William Sellers

Publication

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

A4: S380A, T382A, T383A, S385A

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pY3

Plasmid

#50606

Purpose

Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5

Depositor

Jay Keasling

Publication

Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

Inserts

tyrB
tyrA
aroC
aroA
aroL

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available since

Nov. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJW1310

Plasmid

#61253

Purpose

Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

U6 promoter

Use

CRISPR; Cloning template

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti6.3 GCN1-3xFlag_IRES-iRFP

Plasmid

#198388

Purpose

GCN1 expression

Depositor

Jack Taunton

Publication

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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1437 pSG5L Flag HA PTEN

Plasmid

#10786

Depositor

William Sellers

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN (PTEN Human)

Tags

Flag and HA

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pcDNA3-CCR5

Plasmid

#98943

Purpose

Mammalian expression plasmid for untagged human CCR5

Depositor

Erik Procko

Publication

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CCR5 (CCR5 Human)

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1_iSH2-pMagFast2(3x)-iRFP

Plasmid

#67298

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iSH2-pMagFast2(3x)-iRFP (PIK3R1 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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shRNA-DNMT1i(CDS)

Plasmid

#24950

Depositor

Paul Khavari

Publication

Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

Insert

shRNA DNMT1i CDS

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article