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pSUPER retro puro GFP shRNA

Plasmid

#30519

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

GFP shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNb_opt.IRES.eGFP

Plasmid

#22492

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised wild type Calcineurin B (PPP3R1 Human)

Use

Retroviral

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pEF6-myc-TBC1D15

Plasmid

#79148

Purpose

mammalian expression of TBC1D15

Depositor

Aimee Edinger

Article

Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

Insert

TBC1D15 (Tbc1d15 Mouse)

Tags

His, Myc, and V5

Expression

Mammalian

Mutation

S616N (please see depositor comment below)

Promoter

EF-1alpha

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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NUMB2-eGFP-M107K

Plasmid

#37803

Depositor

Rolf Bjerkvig

Article

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 2 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Promoter

pCMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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mEos2-Mito-7

Plasmid

#57401

Purpose

Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Mito (COX8A Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGBW-m4046828

Plasmid

#145742

Purpose

Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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FU-MAP2-Gateway

Plasmid

#43915

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

V5 and non-standard His tag (HHRHHH)

Expression

Mammalian

Promoter

Human MAP2 promoter

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMi(3xP3-EGFP)

Plasmid

#102540

Purpose

Transgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP marker

Depositor

Michalis Averof, Charalambos Savakis

Article

Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

Insert

Minos transpoable element with 3xP3-EGFP marker

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBSDONR P1-P4

Plasmid

#71744

Purpose

ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Use

Gateway entry vector for use in multisite gateway…

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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977 pSG5L HA PTEN A4

Plasmid

#10753

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

A4: S380A, T382A, T383A, S385A

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pET28CLY9

Plasmid

#21769

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
HNF4A_P2-2200

Plasmid

#31062

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-2200 to -1 of P2 HNF4A promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pF142 pAcGFP1 SOD1 A4V

Plasmid

#26403

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1-3xFlag_IRES-iRFP

Plasmid

#198388

Purpose

GCN1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGH00.0126

Plasmid

#64257

Purpose

Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation

Depositor

Mark Guiltinan, Siela Maximova

Article

Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E

Inserts

EGFP
neomycin phosphotransferase II

Use

Binary agrobacterium vector for plant transformat…

Expression

Plant

Promoter

E12-omega

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-Tev

Plasmid

#177142

Purpose

Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

Tev

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-puro-2A-TEVp

Plasmid

#99610

Purpose

Lentivirus expressing puromycin resistance gene and TEV protease

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

Puromycin resistance (PAC gene)
TEV protease

Use

Lentiviral

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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MIGS2.1 (FF570)

Plasmid

#35247

Purpose

Expression of miR173 precursor under UBQ10 promoter; includes miR173 target site after 35S promoter (Basta selection marker)

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Expression

Plant

Promoter

CaMV 35S

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pQCXIP-LIP

Plasmid

#15714

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LIP (CEBPB Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pTriEx-mPA-GFP-PA-Rac1

Plasmid

#22022

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6X His and mPA-GFP

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only