162,981 results

1. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

   Plasmid

   #104495

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, and AAV1

   Insert

   jGCaMP7s
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-GINKO2

   Plasmid

   #177116

   Purpose

   Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

   Depositor

   Robert Campbell

   Article

   Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

   Insert

   GINKO2
   

   Use

   Tags

   6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. plenti-UBC- IRF4-3xHA-pGK-PUR

   Plasmid

   #107386

   Purpose

   Lentiviral expression of IRF4-3xHA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IRF4 (IRF4 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF1477

   Plasmid

   #143121

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   VAX1 (VAV1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-EGR1

   Plasmid

   #192913

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   EGR1 (EGR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA12-pHR-SFFV-BFP-B23

   Plasmid

   #199443

   Purpose

   Expression of BFP-B23 in mammalian cells

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   B23 (NPM1 Human)
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

   Plasmid

   #200641

   Purpose

   Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

   Depositor

   Justin English, Fleur Ferguson

   Article

   Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

   Insert

   miniTurboID-6xGGSG linker-FKBPF36V-2xHA
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-neo Fluc EGFP

   Plasmid

   #90170

   Purpose

   EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

   Depositor

   Franz-Ulrich Hartl

   Article

   Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

   Insert

   FLuc EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MT-cdh1

    Plasmid

    #11595

    Depositor

    Marc Kirschner

    Article

    Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

    Insert

    cdh1 (FZR1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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