We narrowed to 1,472 results for: piggyBac
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Plasmid#218234PurposeA piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.DepositorTypeEmpty backboneUseCRISPRAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only
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FLAG-FullLength-TET1-WT(21)
Plasmid#124396PurposeFull-length TET1 used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 (TET1 Human)
TagsFlagExpressionMammalianMutationfull length protein is 2136 aaAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6f
Plasmid#62808PurposepPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6f-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TetR-tdTomato
Plasmid#212958PurposepiggyBac donor vector containing TetR-tdTomato for random integration by PBaseDepositorInsertTetR-tdTomato
UseMouse Targeting; Piggybase integration (transposo…TagstdTomatoExpressionMammalianAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.19_PB-CIT-PGK-Blast
Plasmid#205564PurposeExpress human CITDepositorAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB_EF1a-DjCas13d-msfGFP-Blast
Plasmid#226008PurposeDjCas13d protein for RNA targeting in piggyBac backboneDepositorInsertDjCas13d RNA-targeting nuclease
UseCRISPRTagsmsfGFPExpressionMammalianAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-Zim3-MeCP2
Plasmid#220143Purposedoxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repressionDepositorInsertdCas9-Zim3-MeCP2
UseCRISPR and Synthetic Biology; PiggybacExpressionMammalianPromoterTre-3GAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterCAGAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
Plasmid#84239PurposeExpresses ABA-inducible VPR-Sp dCas9DepositorInsertsABI-tagBFP-Sp dCas9
PYL1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…ExpressionMammalianPromoterCAG and PGKAvailable SinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9
Plasmid#84240PurposeExpresses GA-inducible VPR-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…ExpressionMammalianPromoterCAG and PGKAvailable SinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only