We narrowed to 15,050 results for: pET

1. pET28a-MBP-(CBX1)3-eReader

   Plasmid

   #135090

   Purpose

   Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered x3
   

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-His6-Ubc9-C138A

   Plasmid

   #133911

   Purpose

   Bacterial expression of His6-Ubc9-C138A

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Cysteine 138 to Alanine

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-DEST42-SARA-V5-His6

   Plasmid

   #67431

   Purpose

   Expression of hSara with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SARA (SAR1B Human)
   

   Tags

   His and V5

   Expression

   Bacterial

   Promoter

   T7/lacO

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET-FLAG-eSpCas9-plus

   Plasmid

   #126774

   Purpose

   Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-PLS-KP6-A66S_rpoA-U

   Plasmid

   #190969

   Purpose

   Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:A66S

   Promoter

   T7

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-PLS-KP6-E67A_rpoA-U

   Plasmid

   #190973

   Purpose

   Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:E67A

   Promoter

   T7

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-JNKsub(TA)DD-YPet-NES

   Plasmid

   #84634

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   JNKsub(TA)DD-YPet-NES
   

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b-Fab1 (Francisella tularensis)

   Plasmid

   #61690

   Purpose

   Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

   Depositor

   Michael Johnson

   Article

   Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

   Insert

   enoyl-[acyl-carrier-protein] reductase (NADH)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET-pro-siRNA-V2-LMNA

   Plasmid

   #111088

   Purpose

   pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

   Depositor

   Linfeng HUANG

   Article

   Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

   Insert

   Lamin A/C (LMNA Human)
   

   Expression

   Bacterial

   Available Since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-TAF4(827-909)

    Plasmid

    #105620

    Purpose

    bacterially express TAF4 HFD

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF4-HFD(827-909) (Taf4 Mouse)
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

    Plasmid

    #184406

    Purpose

    yeast surface display of the SARS-CoV-2 Beta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    K417N+E484K+N501Y

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3294_pETcon-SARS2-RBD_Omicron-BA1

    Plasmid

    #184408

    Purpose

    yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pet22b-mCherry-TEAD4-IDR

    Plasmid

    #166442

    Purpose

    Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, TEAD4-IDR (TEAD4 Human)
    

    Expression

    Bacterial

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b/GgVcl V1-851 (aka Vh)

    Plasmid

    #46172

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-851 (VCL Chicken)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-851 (aka Vh) head domain

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28A-p53 (1-73) (P12A P13A)

    Plasmid

    #62066

    Purpose

    Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P12A, P13A

    Promoter

    T7

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET-CH2 (1192-1318)

    Plasmid

    #99338

    Purpose

    Bacterial expression of mouse CBP CH2 domain

    Depositor

    Peter Wright

    Article

    Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

    Insert

    CBP CH2 (1192-1318) (Crebbp Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET11a-DAAT-V33G/T242G

    Plasmid

    #159278

    Purpose

    Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

    Depositor

    Roberto Chica

    Article

    Parmeggiani et al ACS Catalysis 9, 3482–3486.

    Insert

    Engineered d-alanine aminotransferase
    

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Valine 33 and threonine 242 have been mutated to …

    Promoter

    T7

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM6-At4CL-PhCHS-MsCHI

    Plasmid

    #73392

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    MsCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21a-mAIM2-deltaPYD-GST

    Plasmid

    #51534

    Purpose

    Express GST-tagged HIN domain of mAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Tags

    GST and T7

    Expression

    Bacterial

    Promoter

    lac operator

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM6-Vv4CL-CmCHS-MsCHI

    Plasmid

    #73402

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Vv4CL
    CmCHS
    MsCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETDuet RagA-RagC(L91P)

    Plasmid

    #99647

    Purpose

    Bacterial expression of RagA-RagC(L91P)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    L91P

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HOXA9 (murine) FLAG pET

    Plasmid

    #8494

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA9 (Hoxa9 Mouse)
    

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    FLAG epitope tag replaces T7 tag in the pET back…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pET151-Vta1(280-330)

    Plasmid

    #87738

    Purpose

    Bacterial expression of the VSL domain of S. cerevisiae Vta1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-279

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-28a(+)-MVK jannaschii

    Plasmid

    #108948

    Purpose

    heterologous expression of MVK of M. jannaschii in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Mevalonate kinase (MVK)
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid

    #138208

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub(T/A)-YPet-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-22HT-MSI1(7-103)

    Plasmid

    #60357

    Purpose

    Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-103) (Msi1 Mouse)
    

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    contains amino acids 7-103 of Msi1

    Promoter

    T7

    Available Since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pET-DEST42-SAR1-V5-His6

    Plasmid

    #67430

    Purpose

    Expression of hSar1 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Tags

    His and V5

    Expression

    Bacterial

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pET22b-ecDHFR-N23PP/G51PEKN

    Plasmid

    #118583

    Purpose

    Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, G51 mutated to P51, a Pro res…

    Promoter

    T7

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET27b-ecDHFR-N23PP/S148A

    Plasmid

    #110829

    Purpose

    Bacterial expression of E.coli DHFR N23PP/S148A mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-Hisx6-Sirt6-S56A

    Plasmid

    #20278

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Serine 56 to Alanine

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only