We narrowed to 4,345 results for: Ado;

1. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

   Plasmid

   #156414

   Purpose

   kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   EGFP

   Expression

   Mutation

   mutant KRAS4B-G12V

   Promoter

   kdrl

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXPR_BRD003 PSMB5 guide 2

   Plasmid

   #117074

   Purpose

   single guide RNA targeting PSMB5; guide 2

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   LMPX (PSMB5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28 MBP-TEV-Rat BCKDK-His

   Plasmid

   #232119

   Purpose

   For bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   BCKDK (Bckdk Rat)
   

   Use

   Tags

   6 x His, Maltose-Binding Protein (MBP), and Tev S…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-PB2_Lock1-I436C+S993C

   Plasmid

   #182879

   Purpose

   Lentivirus for expression of Plexin-B2 locked ring mutant

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

   Insert

   hPLXNB2 (PLXNB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   changed Isoleucine 436 to Cysteine and Serine 993…

   Promoter

   Available since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-PB2_Lock2-I436C+T1051C

   Plasmid

   #182880

   Purpose

   Lentivirus for expression of Plexin-B2 locked ring mutant

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

   Insert

   hPLXNB2 (PLXNB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   changed Isoleucine 436 to Cysteine and Threonine …

   Promoter

   Available since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter

   Plasmid

   #172598

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-D842V (PDGFRA Synthetic, Human)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-D842V

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter

   Plasmid

   #172599

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-D842V+R914W

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter

   Plasmid

   #172600

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-K627R+R914W (PDGFRA Synthetic, Human)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-K627R+R914W

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

   Plasmid

   #172597

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-K627R (PDGFRA Synthetic, Human)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-K627R

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPHR-mCherry-PGL-1-mHP1alpha

    Plasmid

    #181894

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPHR-mCherry-nE-mHP1alpha

    Plasmid

    #181890

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid

    #171803

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-gB-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPaint-mScarlet-F-Ova-BlastR [M1G]

    Plasmid

    #171817

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-Ova-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPaint-mScarlet-F-gB-BlastR [M1G]

    Plasmid

    #171816

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-gB-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPaint-mScarlet-F-hgp100-BlastR [M1G]

    Plasmid

    #171815

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-hgp100-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

    Plasmid

    #171812

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-gB-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPaint-mScarlet-F-mgp100-PuroR [M1G]

    Plasmid

    #171811

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-mgp100-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

    Plasmid

    #171810

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-hgp100-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPaint-mNeon-F-Ova-BlastR [M1G]

    Plasmid

    #171808

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-F-Ova-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-mNeon-F-gB-BlastR [M1G]

    Plasmid

    #171807

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.M1G

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-F-gB-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge