We narrowed to 2,333 results for: Efs

1. pQE-WT-KIF-R5-KIKR

   Plasmid

   #63624

   Purpose

   KIF-R5-KIKR RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   KIF-R5-KIKR RNAP
   

   Use

   Tags

   Expression

   Mutation

   Q744K, L749I, M750K, Q754S, R756N, I761V, H772R

   Promoter

   T5/Lac

   Available since

   April 15, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pQE-WT-N4-R5-YRNRV

   Plasmid

   #63625

   Purpose

   N4-R5-YRNRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   N4-R5-YRNRV RNAP
   

   Use

   Tags

   Expression

   Mutation

   N671Y, L747I, N748D, L749C, M750V, F751I, Q754T, …

   Promoter

   T5/Lac

   Available since

   June 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pAWp30

   Plasmid

   #73857

   Purpose

   pFUGW-EFSp-Cas9-P2A-Zeo

   Depositor

   Timothy Lu

   Article

   Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

   Insert

   EFSp-Cas9-P2A-Zeo
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACYC.Taq.1Amb

   Plasmid

   #102794

   Purpose

   Expresses Taq DNA polymerases with a premature stop codon

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   Taq DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   W168Stop; *(see below)

   Promoter

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE-WT-CGG-R12-KIRV

   Plasmid

   #63668

   Purpose

   CGG-R12-KIRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   CGG-R12-KIRV RNAP
   

   Use

   Tags

   Expression

   Mutation

   Q744K, L747V, N748H, L749I, R756E, L757M, H772R, …

   Promoter

   T5/Lac

   Available since

   April 15, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pQE-WT-CTGA-R13-AKSIRV

   Plasmid

   #63627

   Purpose

   CTGA-R13-AKSIRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   CTGA-R13-AKSIRV RNAP
   

   Use

   Tags

   n/a

   Expression

   Mutation

   V725A, Q744K, L747I, N748S, L749I, R756T, Q758K, …

   Promoter

   T5/Lac

   Available since

   June 3, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pQE-WT-CTGA-R13-AKSRV

   Plasmid

   #63622

   Purpose

   CTGA-R13-AKSRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   CTGA-R13-AKSRV RNAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …

   Promoter

   T5/Lac

   Available since

   July 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pMBP-AsCas12a

   Plasmid

   #113430

   Purpose

   Expresses 10xHis-MBP fused to AsCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AsCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMBP-FnCas12a

   Plasmid

   #113432

   Purpose

   Expresses 10xHis-MBP fused to FnCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   FnCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRST11B-AS3_4

    Plasmid

    #102789

    Purpose

    Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Inserts

    tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
    suppressor tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pMBP-AaCas12b

    Plasmid

    #113433

    Purpose

    Expresses Expresses 10xHis-MBP fused to AaCas12b

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AaCas12b
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMBP-LbCas12a D832A

    Plasmid

    #113517

    Purpose

    Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    D832A

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMBP-LbCas12a H759A

    Plasmid

    #113518

    Purpose

    Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    H759A

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-ER-rsTagRFP

    Plasmid

    #87620

    Purpose

    Endoplasmic reticulum (ER)-targeted rsTagRFP.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    ER-rsTagRFP
    

    Use

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Venus-CAAX

    Plasmid

    #87612

    Purpose

    Plasma membrane-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-LifeAct-Venus

    Plasmid

    #87613

    Purpose

    Actin-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-Venus
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FKBP-Venus-173-C

    Plasmid

    #87617

    Purpose

    FKBP-tagged C-terminal Venus (YFP) fragment for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    FKBP-Venus173-C
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TU#1450_unc-53a_cDNA

    Plasmid

    #82361

    Purpose

    express unc-53a cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-53 a isoform (unc-53 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TU#1448_unc-73b_cDNA

    Plasmid

    #82362

    Purpose

    express unc-73b cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-73 b isoform (unc-73 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge