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pTol1-UAS:ChrimsonR-tdTomato

Plasmid

#124231

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChrimsonR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA

Plasmid

#63931

Purpose

Empty AAV-RAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Promoter

pRAM and TRE

Available Since

Dec. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHR CD19 tandem CAR

Plasmid

#113019

Purpose

for lentivirus production of CD19 tandem CAR

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Synthetic, Mouse, Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA

Plasmid

#84474

Purpose

pAAV-RAM-NLS-mKate2

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

mKate2

Use

AAV

Tags

Nuclear localization signal of SV40 large T antig…

Promoter

TRE

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mEGFP-CHMP2B

Plasmid

#115329

Purpose

expresses mEGFP-CHMP2B in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFE-Selo-FI-prk

Plasmid

#162698

Purpose

Expresses S. elongatus Form IB rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

S. elongatus Form IB rubisco and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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PyronicSF/pcDNA3.1(-)

Plasmid

#124812

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Insert

PyronicSF

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-Rrub-FII-prk

Plasmid

#162695

Purpose

Expresses R. rubrum Form II rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Inserts

Rubrum Form II rubisco
S. elongatus PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter and under same promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:GtACR1-tdTomato

Plasmid

#124235

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR1-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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LZ10 PBREBAC-H2BHalo

Plasmid

#91564

Purpose

piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

Depositor

James Zhe Liu

Article

Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

Insert

histone H2B-HaloTag

Tags

HaloTag

Expression

Mammalian

Promoter

EF1

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:GtACR2-tdTomato

Plasmid

#124236

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR2-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Lamp1-mCherry-FRB

Plasmid

#186576

Purpose

Expression of mCherry-FRB at the outer surface of the lysosome using LAMP1

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

LAMP1 (LAMP1 Human)

Tags

mCherry-FRB

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

Plasmid

#84472

Purpose

pAAV-RAM-ArchT

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

ArchT

Use

AAV

Tags

EGFP

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA

Plasmid

#84468

Purpose

pAAV-CRAM-tdTomato

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

tdTomato

Use

AAV

Mutation

T230I (please see depositor comments below)

Promoter

TRE

Available Since

Dec. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

Plasmid

#84471

Purpose

pAAV-RAM-ChR2

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Has Service

AAV8

Insert

Channelrhodopsin 2

Use

AAV

Tags

EYFP

Mutation

H134R

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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FKBP-mCherry

Plasmid

#108122

Purpose

Recruitable fluorophore

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Plasmid

#175777

Purpose

pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TMEM192 (TMEM192 Human)

Use

CRISPR

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

Plasmid

#84467

Purpose

Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Promoter

pRAM and TRE

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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PyronicSF-mRuby2/pBI-CMV1

Plasmid

#124830

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Inserts

PyronicSF
mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-CALCOCO2

Plasmid

#175759

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-CALCOCO2/NDP52

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

NDP52/CALCOCO2 (CALCOCO2 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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