We narrowed to 5,211 results for: golden gate

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pYPQ132B-CsALSgR1.1

Plasmid

#245875

Purpose

Golden gate entry vector carrying the 1st gRNA for base editing in Carrizo citrange ALS gene

Depositor

Yiping Qi

Publication

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

CsALS_gRNA1.1

Use

CRISPR; Golden gate entry vector to expressing t…

Expression

Plant

Promoter

AtU3

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ133B-CsALSgR1.2

Plasmid

#245876

Purpose

Golden gate entry vector carrying the 2nd gRNA for base editing in Carrizo citrange ALS gene

Depositor

Yiping Qi

Publication

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

CsALSgR1.2

Use

CRISPR; Golden gate entry vector

Expression

Plant

Promoter

AtU3

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCAV035_mCherry_P2A_fsEGFP_GGsite

Plasmid

#219807

Purpose

Fluorescent Reporter for MUTYH-Specific Activity; Containing BsaI Recognition Sequence at Frameshifted EGFP; For OG:A Oligo Insertion at Golden Gate (GG) Site at Codon 34

Depositor

Alexis Komor

Publication

Vasquez et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-sgStuffer-GFP-Puro

Plasmid

#208349

Purpose

Lentivirus sgRNA backbone with GFP and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)

Depositor

Peter S Nelson

Publication

Frank et al eLife 2025

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

hU6

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-sgStuffer-mCherry-Puro

Plasmid

#208350

Purpose

Lentivirus sgRNA backbone with mCherry and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)

Depositor

Peter S Nelson

Publication

Frank et al eLife 2025

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

hU6

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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LLP1003

Plasmid

#239925

Purpose

sgRNA compatible with TCTP-SynPro-07 and CaMV 35S-SynPro-08 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pMK024

Plasmid

#236571

Purpose

Cloning vector for PaqCI Golden Gate assembly of ISAam1 guides into ISAam1 plant expression vector

Depositor

Steven Jacobsen

Publication

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW121_WT-Cas9_U6-entry

Plasmid

#244818

Purpose

Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Publication

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Promoter

CAG, U6

Available since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW493_U6-sgRNA-entry

Plasmid

#244823

Purpose

Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

Depositor

Jennifer Doudna

Publication

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 single guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1011

Plasmid

#239933

Purpose

dCas9 without Stop codon as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

dCas9

Use

CRISPR and Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1004

Plasmid

#239926

Purpose

sgRNA compatible with TCTP-SynPro-07 and CaMV 35S-SynPro-12 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1005

Plasmid

#239927

Purpose

sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1006

Plasmid

#239928

Purpose

sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1001

Plasmid

#239923

Purpose

sgRNA compatible with TCTP-SynPro-09 and CaMV 35S-SynPro-03 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1002

Plasmid

#239924

Purpose

sgRNA compatible with TCTP-SynPro-09 and CaMV 35S-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP996

Plasmid

#239918

Purpose

sgRNA compatible with TCTP-SynPro-05 and CaMV 35S-SynPro-15 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP997

Plasmid

#239919

Purpose

sgRNA compatible with TCTP-SynPro-05 and CaMV 35S-SynPro-07 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP999

Plasmid

#239921

Purpose

sgRNA compatible with TCTP-SynPro-08 and CaMV 35S-SynPro-04 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1000

Plasmid

#239922

Purpose

sgRNA compatible with TCTP-SynPro-08 and CaMV 35S-SynPro-04 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1008

Plasmid

#239930

Purpose

sgRNA compatible with TCTP-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge