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CapChR2_pUAST-attb

Plasmid

#188041

Purpose

Expresses CapChR2 in Drosophila

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2

Use

Tags

none

Expression

Insect

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

UAS

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mito-MiCy

Plasmid

#174177

Purpose

Express MiCy with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

MiCy with mitochondria-targeting sequence

Use

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-Venus

Plasmid

#175828

Purpose

Express Venus with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with microtuble-targeting sequence

Use

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-mCherry

Plasmid

#175822

Purpose

Express mCherry with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMB

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-iRFP

Plasmid

#175830

Purpose

Express iRFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with microtuble-targeting sequence

Use

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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S12.1_Empty_Bridge

Plasmid

#127670

Purpose

Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAB088c

Plasmid

#79213

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-TnTBR3-F3

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1); Ppro1

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-RCK

Plasmid

#25065

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the ROC-COR-Kinase domains aa 1329…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pBXNH3LCA_TM287/288

Plasmid

#132701

Purpose

expresses TM287/288 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

TM287/288 (TM0288, TM0287 Thermotoga maritima)

Use

Tags

Avi and His

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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p6604 MSCV-IP N-HAonly PP2CA

Plasmid

#35005

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

PP2CA (PP2CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-nef-CIAO-TVA-mCherry

Plasmid

#149295

Purpose

Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

TVA:mCherry

Use

AAV

Tags

Expression

Mutation

Start codon removed.

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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S12.2_Bridge + Target

Plasmid

#127671

Purpose

Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFX-Venus-Sec61β

Plasmid

#174467

Purpose

Express Venus with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-EGFP

Plasmid

#175839

Purpose

Express EGFP with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with nucleus-targeting sequence

Use

Tags

H2B (human histone 2B)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBS DKK3

Plasmid

#19201

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Dickkopf homolog 3 (DKK3 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pFX-EGFP-KRasCT

Plasmid

#175833

Purpose

Express EGFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with plasma membrane-targeting sequence

Use

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pOCC193

Plasmid

#118903

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette

Use

Tags

MBP, cleavable with 3C protease; mGFP and TwinStr…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC305

Plasmid

#118898

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette

Use

Tags

HIS6, cleavable with 3C protease and TwinStrep-MB…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-Syk-tSH2

Plasmid

#111269

Purpose

express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-Sec61β

Plasmid

#174468

Purpose

Express mCherry with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge