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pKD280.7

Plasmid

#173172

Purpose

Expresses SO_4387

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

SO_4387

Expression

Bacterial

Promoter

J23107

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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prp8-G736E

Plasmid

#108450

Purpose

prp8-G736E in pRS313 backbone for expression in yeast

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

changed G736 to E

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pFvL118

Plasmid

#64752

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-GOS1

Plasmid

#18846

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

GOS1 (GOS1 Budding Yeast)

Tags

cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-myrSNAP-CON

Plasmid

#60099

Purpose

Expresses membrane-targeted SNAP(f)-tag and histone-GFP, conditional

Depositor

Gregory Jefferis

Article

Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

Inserts

SNAP-tag
histone-GFP

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pTW081

Plasmid

#64755

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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3579 pcDNA3 Bad S136A

Plasmid

#8798

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

S136A

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pGEX-6p-hDcp2

Plasmid

#72216

Purpose

bacterial expression of GST-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

GST

Expression

Bacterial

Mutation

F146Y and E198K (please see depositor's comm…

Promoter

tac

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pBT344_pattB-pCA-GFP-pA-FRT5

Plasmid

#52550

Purpose

for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTY153 EGFP-T2A-ORF6 SARS-CoV-2

Plasmid

#204975

Purpose

Co-express EGFP and SARS-CoV-2 ORF6

Depositor

Tim Mitchison

Article

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-2)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_MCS_version2

Plasmid

#197961

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-vCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_MCS_version2

Plasmid

#197962

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-nCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTY152 EGFP-T2A-ORF6 SARS-CoV-1

Plasmid

#204974

Purpose

Co-express EGFP and SARS-CoV-1 ORF6

Depositor

Tim Mitchison

Article

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-1)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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nuc-yHS1

Plasmid

#159166

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1

Tags

SV40 NLS

Expression

Yeast

Promoter

GPD

Available Since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Cyan Nano-lantern/pRSETB

Plasmid

#67658

Purpose

cyan luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pBSFI-myr-akt-T308A

Plasmid

#49189

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

T308A

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-PAX9

Plasmid

#75079

Purpose

retroviral vector holding human PAX9 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pmiReport SHIP1 3' UTR

Plasmid

#26531

Depositor

David Baltimore

Article

O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

Insert

SHIP1 3'UTR (Inpp5d Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pLL3.7 shRNA against CRTC2

Plasmid

#72711

Purpose

Lentiviral expression of shRNA against CRTC2 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC2

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only