We narrowed to 9,067 results for: sgRNA

Showing: 2161 - 2180 of 9067 results

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pEPOR1CB0081

Plasmid

#117561

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtMPK19 {AT3G14720} in A. thaliana

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_AtMPK19 {AT3G14720} A. thaliana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Publication

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

Plasmid

#126778

Purpose

Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HiFi SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R691A

Promoter

CBh

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Publication

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Cas12c-sgRNA_BbsI_CAM

Plasmid

#183221

Purpose

Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Publication

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c single guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-2XPP7

Plasmid

#194504

Purpose

Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

Depositor

Chunqing Song

Publication

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-PEX3

Plasmid

#227303

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA Targeting C-terminus of PEX3 (PEX3 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-RAB7A

Plasmid

#227297

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA Targeting N-terminus of RAB7A (RAB7A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-CEP135

Plasmid

#227286

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA Targeting N-terminus of CEP135 (CEP135 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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px459-H3.3 (H3f3b) sgRNA

Plasmid

#186931

Purpose

H3f3b sgRNA used for genomic targeting at H3f3b C-terminal

Depositor

Zhiguo Zhang

Publication

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3b sgRNA (H3f3b Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#162334

Purpose

Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

Depositor

Charles Gersbach

Publication

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSaCas9-KRAB

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hUbC

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9658-sgRNA-mCherry

Plasmid

#162987

Purpose

sgRNA cloning plasmid

Depositor

Ervin Welker

Publication

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

exchangeable cassette

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

Plasmid

#218528

Purpose

sgRNA targeting human EIF2AK4/GCN2

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK4 (EIF2AK4 Human)

Use

CRISPR and Lentiviral

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::axed{sgRNA})

Plasmid

#187883

Purpose

Gal4/UAS sgRNA expression targeting axed

Depositor

Lukas Neukomm

Publication

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting axed

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::hiw{sgRNA})

Plasmid

#187884

Purpose

Gal4/UAS sgRNA expression targeting hiw

Depositor

Lukas Neukomm

Publication

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting hiw

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::dsarm{sgRNA})

Plasmid

#187885

Purpose

Gal4/UAS sgRNA expression targeting dsarm

Depositor

Lukas Neukomm

Publication

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting dSarm

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ECT2_sgRNA

Plasmid

#183875

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0021

Plasmid

#117557

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0020

Plasmid

#117556

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0019

Plasmid

#117555

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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