We narrowed to 2,097 results for: PAM

1. xCas9 3.7

   Plasmid

   #108379

   Purpose

   Mammalian expression vector for xCas9 3.7

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.7
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

   Plasmid

   #194063

   Purpose

   Mammalian expression of evoCjCas9 PEmax_deltaRnH

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-PEmax_dRnH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Nme2Cas9_pMCSG7

   Plasmid

   #119925

   Purpose

   Nme2Cas9 bacterial expression plasmid

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   Tags

   NLS, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCnCas12f1HS_sgRNA_MS13_empty

   Plasmid

   #204999

   Purpose

   CnCas12f1 with MS13 sgRNA site for Human cell genome editing

   Depositor

   Quanjiang Ji

   Article

   Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-PE2-SpRY-P2A-GFP

   Plasmid

   #159981

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY-P2A-GFP
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p2T-CMV-evoCjCas9-ABE8e-BlastR

   Plasmid

   #194060

   Purpose

   Mammalian expression of evoCjCas9 ABE8e

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTW211b5

   Plasmid

   #136927

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9(N)-NpuN
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   see manuscript

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-P3-evoCjCas9-ABE8e_gRNA

   Plasmid

   #202559

   Purpose

   P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-eNme2-C.NR

   Plasmid

   #185672

   Purpose

   Expresses nuclease active eNme2-C.NR in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   eNme2-C.NR nuclease
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-PE2-SpG-P2A-GFP

    Plasmid

    #159980

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpG-P2A-GFP
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a_CnCas12f1

    Plasmid

    #204996

    Purpose

    CnCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

    Insert

    CnCas12f1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Cas9-NRCH

    Plasmid

    #136926

    Purpose

    Expresses Cas9-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEND-418_Cacnes_LysA

    Plasmid

    #225607

    Purpose

    Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Nme2sgRNA_pLKO

    Plasmid

    #119926

    Purpose

    Nme2Cas9 U6-driven sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    NmeCas9 sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. xCas9 3.6

    Plasmid

    #108384

    Purpose

    Mammalian expression vector for xCas9 3.6

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9 3.6
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-SlugABE-NNG

    Plasmid

    #214356

    Purpose

    Expresses SlugABE-NNG

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p2T-CMV-evoCjCas9-BE4max-BlastR

    Plasmid

    #194061

    Purpose

    Mammalian expression of evoCjCas9 CBE4max

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-hSyn-evoCjCas9-ABE8e_gRNA

    Plasmid

    #202561

    Purpose

    hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Cas9-NRRH

    Plasmid

    #136924

    Purpose

    Expresses Cas9-NRRH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRRH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAGK112

    Plasmid

    #228127

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MSP1594

    Plasmid

    #65775

    Purpose

    Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-SpCas9-P2A-puro

    Plasmid

    #214358

    Purpose

    Expresses SpCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAGK111

    Plasmid

    #228126

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SchBE4max

    Plasmid

    #192135

    Purpose

    Expresses AncBE4max and nSchCas9

    Depositor

    Yongming Wang

    Article

    Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

    Insert

    AncBE4max, nSchCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SchCas9 (D9A)

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSP2283

    Plasmid

    #70702

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCALNL-eGFP-NT1-6-gix-6-NT1

    Plasmid

    #81204

    Purpose

    contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEND-443_Cacnes_HisB

    Plasmid

    #225608

    Purpose

    Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MP6-SlugABEN-738

    Plasmid

    #214348

    Purpose

    Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-SpCas9HF1-P2A-puro

    Plasmid

    #214357

    Purpose

    Expresses SpCas9HF1 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9HF1 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-SlugCas9-P2A-puro

    Plasmid

    #214353

    Purpose

    Expresses SlugCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Nme2Cas9_AAV

    Plasmid

    #119924

    Purpose

    All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    AAV

    Tags

    NLS-3xHA-NLS and NLS-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEND-349_Cacnes_ori

    Plasmid

    #225604

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEND-320_Cacnes_ori

    Plasmid

    #225602

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEND-321_Cacnes_ori

    Plasmid

    #225603

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEND-350_Cacnes_ori

    Plasmid

    #225605

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. xCas9(3.7)-BE4

    Plasmid

    #108381

    Purpose

    Mammalian expression vector for xCas9(3.7)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-BE4
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. xCas9(3.7)-BE3

    Plasmid

    #108380

    Purpose

    Mammalian expression vector for xCas9(3.7)-BE3

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-BE3
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-SlugCas9-NNG-P2A-puro

    Plasmid

    #214352

    Purpose

    Expresses SlugCas9-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pEND-400_Cacnes_CAMP2

    Plasmid

    #225606

    Purpose

    Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCMV-SlugCBE-NNG-P2A-puro

    Plasmid

    #214355

    Purpose

    Expresses SlugCBE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCBE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. MSP2292

    Plasmid

    #70707

    Purpose

    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    T7

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSFFV-dNme2Cas9

    Plasmid

    #193190

    Purpose

    Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    dNme2Cas9
    

    Use

    Tags

    NLS and NLS-3xHA-NLS

    Expression

    Mammalian

    Mutation

    H588A, D16A

    Promoter

    SFFV

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pEND-193_Cacnes_HS

    Plasmid

    #225622

    Purpose

    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    HspR
    

    Use

    Synthetic Biology

    Tags

    pFAST-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEND-192_Cacnes_HS

    Plasmid

    #225621

    Purpose

    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    HspR
    

    Use

    Synthetic Biology

    Tags

    pFAST-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. xCas9(3.6)-BE4

    Plasmid

    #108386

    Purpose

    Mammalian expression vector for xCas9(3.6)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-BE4
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge