Addgene

We narrowed to 350 results for: HDR;

Showing: 201 - 220 of 350 results

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pJAT58

Plasmid

#204304

Purpose

Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-DsRed

Use

CRISPR

Tags

Expression

Mutation

Promoter

3XP3

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT71

Plasmid

#204305

Purpose

Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-DsRed

Use

CRISPR

Tags

Expression

Mutation

Promoter

ie1

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pYC-C

Plasmid

#207114

Purpose

HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

Depositor

Yifan Cheng

Article

Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pYC-N

Plasmid

#207113

Purpose

HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

Depositor

Yifan Cheng

Article

Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)

Plasmid

#179078

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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BFP Homology Donor

Plasmid

#149372

Purpose

Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Insert

CDDR BFP Homology Donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR)

Plasmid

#178987

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)

Plasmid

#179079

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-PtH2B

Plasmid

#133390

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

No promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

Plasmid

#178985

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

Plasmid

#178989

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-CPspaH2B

Plasmid

#133385

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-PtH

Plasmid

#133391

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-C3HTK

Plasmid

#133388

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-BtH

Plasmid

#133392

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK09 pCAS-Tyr-[gRNA: BsaI GFP dropout] (BsmBI-flanked URA3, AmpR)

Plasmid

#178993

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = URA3

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)

Plasmid

#178991

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-C3H

Plasmid

#133386

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge