We narrowed to 3,670 results for: MAX
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Plasmid#188314PurposeExpression of eGFP-Luz via a T7 promoter in TXTLDepositorInsertGFP-Luz fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxE
Plasmid#188309PurposeExpression of LuxE via a T7 promoter in TXTLDepositorInsertluxE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV‐ABEmaxNGX‐NGA
Plasmid#187059PurposeABEmax-SpCas9-NGX‐NGADepositorInsertABEmaxNGX‐NGA
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV‐ABEmaxNGX‐NGC
Plasmid#187060PurposeABEmax-SpCas9-NGX-NGCDepositorInsertABEmaxNGX‐NGC
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV‐ABEmaxNGX‐loop
Plasmid#187061PurposeABEmax-SpCas9-NGX-loopDepositorInsertABEmaxNGX‐loop
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 CP ABEMax strategy 2
Plasmid#135358PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)DepositorInsertWT Tada-nSpyCas9 aa 1029-evolved Tada
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 CP ABEMax strategy 3
Plasmid#135359PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminusDepositorInsert1029 CP nSpyCas9 ABEMax
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S2 1058 V82G ABEMax
Plasmid#135361PurposeExpression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9DepositorInsert1058 nSpyCas9 V82G ABEMax
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
miniABEMax V82G nSaCas9
Plasmid#135365PurposeExpression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitutionDepositorInsertminiABEMax V82G nSaCas9
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-EA-BE4max-NG-BlastR
Plasmid#152992PurposeHigh purity C-to-T base editorDepositorInsertBE4 H47E+S48A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-GFP
Plasmid#180020PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-EGFP markerDepositorInsertPEmax-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax
Plasmid#222138PurposeAdenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9 (cas9 Synthetic)
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-ePEmax3::tEURb7
Plasmid#227692PurposeGolden Gate level 1, position 2, ePEmax3 expression cassette.DepositorInsertePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-PE2max-NC::tEURb7
Plasmid#227693PurposeGolden Gate level 1, position 2, PE2max-NC expression cassette.DepositorInsertPE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hMLH1dn
Plasmid#174828PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPEmax-P2A-hMLH1dn
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:: pRPS5a-PE2max-NC::tHSP
Plasmid#227694PurposeGolden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.DepositorInsertPE2max-NC being driven by pRPS5a and terminated by tHSP
UseCRISPRTagsExpressionPlantMutationPromoterpRPS5aAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmax pona 12xTRICK 24xMS2SL
Plasmid#64542PurposeTRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.DepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, HA, NLS, and dNP799ExpressionMammalianMutationPromoterponAAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-YE1-BE4max
Plasmid#198549PurposeExpresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE5max-SF3B1-K700E
Plasmid#200317PurposeExpresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.DepositorInsertsK700E-ng1+pg2
PEmax-2A-MLH1dn
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only