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Plasmid#32965PurposeFor mammalian expression of mouse MEF2D with a 3x FLAG tag on the C-terminus.DepositorInsertMEF2D (Mef2d Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCHOP10.pCDNA1
Plasmid#21899DepositorInsertCHOP10 (Ddit3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTK298
Plasmid#59567PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4DepositorInsertSGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExp_TRE:EBFP2
Plasmid#51793PurposeInducible expression of EBFP2 with rtTA for input fluorescenceDepositorInsertEBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-101-3p
Plasmid#103165PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-101-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-101-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3XFLAG-MKL1-~100
Plasmid#27176DepositorInsertMKL1 (MRTFA Human)
UseTags3XFLAGExpressionMammalianMutationDeletion of 100 aa from ATGPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTK333
Plasmid#59574PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5DepositorInsertSGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-SNAP-Csk)
Plasmid#177895PurposeBaculo expression of His10 tag fused to SNAPtag and human CskDepositorInsertCsk (CSK Human)
UseTags10xHis-TEV-TVMV-SNAP tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28 His10-FcRÉŁ
Plasmid#113021Purposefor purification of His10-FcRÉŁ intracellular domainDepositorInsertHis10-FcRÉŁ intracellular domain
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pMT Stat92E-GFP
Plasmid#126662PurposeInducible expression of Stat92E-GFP in S2 cellsDepositorInsertStat29E
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(His10-PD-1cyto)
Plasmid#177896PurposeE coli expression of His10 tag fused to human PD-1 Intracellular DomainDepositorInsertPD-1 (aa 194-288 only) (PDCD1 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(CTLA4)
Plasmid#177905PurposeBacterial expression of his10-tagged human CTLA4 Intracellular DomainDepositorInsertCTLA4 (aa 183-223 only) (CTLA4 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
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mP58.FL1-pCDNA3
Plasmid#21883DepositorInsertP58 (Dnajc3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pET28a(DAP10)
Plasmid#177904PurposeBacterial expression of his10-tagged human DAP10 Intracellular DomainDepositorInsertDAP10 (aa 70-92 only) (HCST Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-2 SNAP-Syk tSH2
Plasmid#113028Purposefor purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.DepositorInsertSNAP-Syk tSH2
UseTagsSNAPExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
aCE120
Plasmid#204491PurposeFigure 1. reporter circuitDepositorInsertsEBFP2
mKate2
EYFP
UseSynthetic BiologyTagsnoneExpressionBacterial and MammalianMutationPromoterhEF1aAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only