We narrowed to 849 results for: Tg

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pET41a iGluu

Plasmid

#119834

Purpose

Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluu

Tags

GST

Expression

Bacterial

Mutation

S72T

Promoter

T7

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)

Plasmid

#164184

Purpose

Freeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)

Depositor

Thomas Oertner

Article

Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

Inserts

CamPARI2
Human α Tubulin

Use

AAV

Tags

6 His tag and NES

Expression

Mammalian

Promoter

Syn

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-G97 IRES EGFP

Plasmid

#130711

Purpose

Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluSnFR

Plasmid

#106123

Purpose

Indicator for glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR

Tags

Myc and PDGFR

Expression

Mammalian

Promoter

synapsin

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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sFlt1-e15a V5

Plasmid

#86045

Purpose

human Soluble Flt1 isoform e15a (terminates in exon 15a)

Depositor

Christie Thomas

Article

Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

Insert

human soluble Flt1 isoform e15a (FLT1 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

isoform e15a (terminates in exon 15a)

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2d-cs-TEV-STREP

Plasmid

#178912

Purpose

Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d

Tags

C term strep and N term mcherry

Expression

Mammalian

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_dC_mKate2

Plasmid

#167336

Purpose

Expresses mKatet2 fused to TFAM dC-tail mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_linker_mKate2

Plasmid

#167335

Purpose

Expresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_LAMA-N95 (for GFP)

Plasmid

#130716

Purpose

Plasmid encoding the bacterial expression of LAMA-N95 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-N95

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV Syn 2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#165491

Purpose

Neuronal expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity .

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

AAV

Tags

Venus

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP

Plasmid

#184811

Purpose

Used to generate the Tg(QUAS:GFP)c578 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-pA

Use

Zebrafish tol2 transgenesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_dHMGA_pET28

Plasmid

#167329

Purpose

Expresses TFAM dHMGA mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing linker, HMGB, and C-…

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn1 ChR2 ET-TC 2A tDimer

Plasmid

#101361

Purpose

High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

Depositor

Thomas Oertner

Article

Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

Inserts

Channelrhodopsin-2
synaptophysin-red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E123T , T159C

Promoter

human Synapsin

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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bPAC-mycHis_pGEM

Plasmid

#85468

Purpose

Expression of photoactivated cyclase bPAC of Beggiatoa sp.

Depositor

Peter Hegemann

Article

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

Bacterial Photoactivated Adenylyl Cyclase

Tags

His and Myc

Promoter

T7 promoter

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFE-hCA

Plasmid

#132999

Purpose

pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

hCAII (CA2 Human)

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98 IRES EGFP

Plasmid

#130706

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-Aurora

Plasmid

#98167

Purpose

red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Aurora gene

Tags

mCherry

Expression

Mammalian

Mutation

V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

Promoter

CMV (+enhancer)

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE

Plasmid

#166970

Purpose

Cre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.

Depositor

Matthew Banghart

Article

Liu et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

Homo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)

Use

AAV and Cre/Lox

Tags

FLAG, T2A, and mCherry

Expression

Mammalian

Promoter

hsyn

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer

Plasmid

#66779

Purpose

The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

Depositor

Thomas Oertner

Article

Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

Inserts

hbeRhGC
red fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

human Synapsin

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-iC++

Plasmid

#98165

Purpose

artificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct iC++ gene

Tags

mCherry

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, V242R, T24…

Promoter

CMV (+enhancer)

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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