We narrowed to 9,068 results for: mel

1. EGFP-Rab8a-wt

   Plasmid

   #86075

   Purpose

   A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMCL-HA-MAPKK1-WT

   Plasmid

   #40808

   Depositor

   Natalie Ahn

   Article

   Mansour et al Science. 1994 Aug 12;265(5174):966-70.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

3. GST-Rab8a-wt

   Plasmid

   #86078

   Purpose

   A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCL-HA-MAPKK1-8E (K97M)

   Plasmid

   #40811

   Depositor

   Natalie Ahn

   Article

   Mansour et al Science. 1994 Aug 12;265(5174):966-70.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K97M

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

5. TurboID-MEK1

   Plasmid

   #226169

   Purpose

   Used to transiently express a TurboID fusion of the protein kinase MEK1.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   Myc-TurboID-MEK1 (MAP2K1 Human)
   

   Tags

   Myc-TurboID

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTNR1B-DuET

   Plasmid

   #213349

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MTNR1B (MTNR1B Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL3.7m-psMEK1tight

   Plasmid

   #89362

   Purpose

   A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2017 Feb 24;355(6327):836-842.

   Insert

   psMEK1tight (MAP2K1 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Human influenza hemagglutinin (HA) tag and pdDron…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLL3.7m-psMEK1

   Plasmid

   #89361

   Purpose

   A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2017 Feb 24;355(6327):836-842.

   Insert

   psMEK1 (MAP2K1 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Human influenza hemagglutinin (HA) tag and pdDron…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMCL-HA-MAPKK1-11A55B [S218E/S222D]

   Plasmid

   #40809

   Depositor

   Natalie Ahn

   Article

   Mansour et al Science. 1994 Aug 12;265(5174):966-70.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S218E, S222D

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

10. MTNR1A-Tango

    Plasmid

    #66443

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MTNR1A (MTNR1A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET14b His Rab8a Q67L

    Plasmid

    #186014

    Purpose

    His Rab8a Q67L

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    His Rab8a Q67L (RAB8A Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rab8WT(Flag) in pcDNA3.1neo

    Plasmid

    #46783

    Purpose

    expression of WT Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A (RAB8A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

13. MBP-MEK1

    Plasmid

    #68300

    Purpose

    N-terminal MBP tagged human MEK1 gene for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 (MAP2K1 Synthetic, Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-Rab8a-QL

    Plasmid

    #86076

    Purpose

    A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EGFP-Rab8a-TN

    Plasmid

    #86077

    Purpose

    A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed threonine 22 to aspargine

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTNR1B-Tango

    Plasmid

    #66444

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MTNR1B (MTNR1B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMCL-HA-MAPKK1-G1C [delta(44-51)/S218E/S222D]

    Plasmid

    #40807

    Depositor

    Natalie Ahn

    Article

    Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(44-51), S218E, S222D

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

18. L1E-1

    Plasmid

    #21193

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek1 (Esr1, MAP2K1 Mouse, Human)
    

    Use

    Retroviral

    Mutation

    Mek1 R4F fused to ER*, constitutive active

    Available Since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. CC2

    Plasmid

    #21207

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek1 (Esr1, MAP2K1 Mouse, Human)
    

    Use

    Subclone vector

    Mutation

    Mek1 R4F fused to ER*, constitutive active

    Available Since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only

20. DN9

    Plasmid

    #21209

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek1 (Esr1, MAP2K1 Mouse, Human)
    

    Use

    Subclone vector

    Mutation

    Mek1 K97M fused to ER*, catalytically inactive

    Available Since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only