We narrowed to 1,249 results for: vent

1. pWM_12x601_35bpLinker

   Plasmid

   #157790

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_35bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWM_12x601_193 NRL + TetO

   Plasmid

   #157812

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_tetO
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Nuclear Myosin I YFP

   Plasmid

   #60241

   Purpose

   Expresses nuclear isoform of myosin IC attached to YFP and an NLS

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p32Fn

   Plasmid

   #158153

   Purpose

   Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Rice codon optimized

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAS9-TPC

   Plasmid

   #61478

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PcUbi4-2

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZY102

   Plasmid

   #73933

   Purpose

   This plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.

   Depositor

   Zhanyuan Zhang

   Article

   Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

   Inserts

   bar
   GUS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   intron is inserted into the GUS coding region

   Promoter

   35S

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST14-SpyCatcher002 EQ

   Plasmid

   #102830

   Purpose

   Bacterial expression of unreactive version of SpyCatcher002

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002 EQ
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   E77Q mutation prevents isopeptide bond formation

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDV05.1_pEF1a-iRFP720_frameshifted

   Plasmid

   #200408

   Purpose

   Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720 (frameshift mutant)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KG#371

   Plasmid

   #110882

   Purpose

   Expresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neurons

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2013 May;194(1):143-61. doi: 1

   Inserts

   unc-129 promoter
   CTNS-1a-mCherry cDNA
   unc-54 3' control region with 1 intron just upstream and 1 intron in control region
   

   Use

   Tags

   intron-mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid

    #69547

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Expression

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge