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pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC
Plasmid#22931PurposeMammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)DepositorInsertPHLPP1-deltaC (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPHLPP-deltaC, deletion of PDZ binding domain--the…PromoterCMVAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-hM3Dq
Plasmid#114283PurposeTET-inducible expression of the hM3Dq proteinDepositorInserthM3Dq
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLmoCascade-IL1RNcrRNA
Plasmid#126487PurposeEncodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RNDepositorInsertLmo IL1RN cr03
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
TDP43 NOTAG3
Plasmid#28208DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsExpressionMammalianMutationaa170-414 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMA-Thermal_CTLA-4
Plasmid#199751PurposeThermal expression of CTLA-4DepositorInsertCTLA-4 (Ctla4 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-FL-TagBFP
Plasmid#120921PurposeExpresses BFP tagged Ptch1DepositorInsertptch1 (Ptch1 Mouse)
UseTagsTagBFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSI-535
Plasmid#131130PurposegRNA expression vector for C-to-T base editing reporterDepositorInsertC-to-T reporter activation gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
GWF180
Plasmid#133607PurposeCAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9DepositorInsertDNCR2-VPR, NS3aH1-tagBFP-SpdCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG
Plasmid#132682PurposeAAV plasmid encoding mouse PM20D1 with C-terminal flag tagDepositorInsertPeptidase M20 domain containing 1 (Pm20d1 Mouse)
UseAAVTags6xHIS and FlagExpressionMutationPromoterChicken b-actinAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQZ083
Plasmid#106417PurposeExpress Complexin-1 fragment (1-83) in E. coliDepositorInsertcomplexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)
UseTagsGST tagExpressionBacterialMutationFragment 1-83, Codon optimizationPromoterT7Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T merlin 1-332
Plasmid#11631DepositorInsertmerlin (NF2 Human)
UseTagsGSTExpressionBacterialMutationAmino-terminal half of merlin.PromoterAvailable sinceMay 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ
Plasmid#192380PurposeTo test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB3 AurA
Plasmid#68270Purposebacterial expression of human AurADepositorInsertAurA (AURKA Human)
UseTagsMxe intein/chitin binding domainExpressionBacterialMutationSee depositor comment belowPromoterT7Available sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSUPER.retro-{beta}4 SCR
Plasmid#16267DepositorInsertSCR Beta 4 Integrin (ITGB4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-merlin 1-339
Plasmid#168128PurposeBacterial expression of human Nf2/merlin 1-339DepositorInsertMerlin (NF2 Human)
UseTagsGlutathione S-transferase (GST)ExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI101.1/ProGOXL1:GUS
Plasmid#175557PurposeBinary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genesDepositorInsertProGOXL1
UseTagsGUSExpressionPlantMutationPromoterGOXL1Available sinceJan. 13, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJBL704
Plasmid#140374PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoterDepositorInsertT7-4BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EK0313 SFFV-mCherry-SG(s70587)SG-Cre (FLP-IN)
Plasmid#191149PurposeExpression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun LuoDepositorInsertmCherry:s70587:Cre
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-2xFLAG-Atoh1ER-DsRed
Plasmid#101735PurposeExpresses mouse Atoh1 fused to ER and DsRedDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2x FLAG, DsRed, and ERExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-MRPP1_native+
Plasmid#67865Purposebacterial expression of TRMT10C, full coding sequence (mitoch. form)DepositorInsertMRPP1 (TRMT10C Human)
UseTagsExpressionBacterialMutationmitoch. form (contains aa 40-403)PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN-HA-CYLD
Plasmid#27556DepositorInsertCYLD (CYLD Human)
UseRetroviralTagsHAExpressionMammalianMutationR156G and G179RPromoterAvailable sinceAug. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJBL705
Plasmid#140375PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoterDepositorInsertT7-5BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T merlin 340-595
Plasmid#11634DepositorInsertmerlin (NF2 Human)
UseTagsGSTExpressionBacterialMutationCarboxy-terminal half of merlin isoform 1.PromoterAvailable sinceMay 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-CYLD
Plasmid#27547DepositorInsertCYLD (CYLD Human)
UseTagsHAExpressionMammalianMutationR156G and G179RPromoterAvailable sinceAug. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSR-siKlf5
Plasmid#41740DepositorInsertKlf5 (Klf5 Mouse)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)
Plasmid#191177PurposeMammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).DepositorInsertmCherry:GAPDH.s1:EGFP (frameshifted)
UseTagsExpressionMammalianMutationEGFP is frameshiftedPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)
Plasmid#191157PurposeExpression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp longDepositorInsertmTagBFP2:Bdnf.s1:mCherry
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533AK570A
Plasmid#14104DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to Ala, Lys570 to AlaninePromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only
