We narrowed to 28,886 results for: PLE

1. pRS426-GFP-PLCdelta-PH

   Plasmid

   #71375

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   PLCdelta-PH
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain

   Promoter

   Available since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pJJH1295

   Plasmid

   #36914

   Depositor

   Jürgen Heinisch

   Article

   Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

   Insert

   3x mCherry
   

   Use

   Tags

   Expression

   Bacterial, Mammalian, and Y…

   Mutation

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRA03

   Plasmid

   #103141

   Purpose

   Triple gRNA expression separated with 28nt Csy4 motif

   Depositor

   Jens Nielsen

   Article

   Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

   Insert

   gRNAs
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMZ288

   Plasmid

   #59897

   Purpose

   Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAM3558

   Plasmid

   #40252

   Depositor

   Susan Golden

   Article

   Chen et al Plasmid. 2008 May;59(3):176-92. Epub 200

   Insert

   Gm cassette (BssHII+SphI)
   

   Use

   Cyanobacteria cloning vector

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAWp9

   Plasmid

   #73851

   Purpose

   pFUGW-UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}

   Depositor

   Timothy Lu

   Article

   Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

   Insert

   UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAW204

   Plasmid

   #113244

   Purpose

   E. coli expression vector for IHFa F13A + IHFb

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   IHFa F13A (ihfA E. coli)
   IHFb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F13A

   Promoter

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMZ289

   Plasmid

   #59898

   Purpose

   Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAW206

   Plasmid

   #113246

   Purpose

   E. coli expression vector for IHFa K15A + IHFb

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   IHFa K15A (ihfA E. coli)
   IHFb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K15A

   Promoter

   T7

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAW212

    Plasmid

    #113234

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->CCATC

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMZ381

    Plasmid

    #59899

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJJH1242

    Plasmid

    #36909

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Insert

    ScURA3
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-stl

    Plasmid

    #51721

    Purpose

    conditional gene knockdowns in Trypanosoma brucei

    Depositor

    Arthur Gunzl

    Article

    Brandenburg et al EMBO J. 2007 Nov 28;26(23):4856-66. Epub

    Type

    Empty backbone

    Use

    Conditional gene knockdown in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    T7 (2X)

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJJH1338

    Plasmid

    #36912

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Insert

    ScURA3
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAWp9-3

    Plasmid

    #73854

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Insert

    UBCp-RFP-CMVp-GFP-U6p-RFP-sg2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pAWp9-1

    Plasmid

    #73852

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-U6p-GFP-sg1

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Insert

    UBCp-RFP-CMVp-GFP-U6p-GFP-sg1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pJJH1307

    Plasmid

    #36910

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Insert

    ScLEU2
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Hv_HPT:pCSRM2C:Gus_introns

    Plasmid

    #215191

    Purpose

    Evaluating guard cell specific promotors in Barley

    Depositor

    Penny Hundleby

    Article

    Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

    Insert

    Scream2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    BdSCRM2

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SJZ6-HIS5

    Plasmid

    #228259

    Purpose

    Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    his5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 S.c.

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSliceRep_B_miR-7

    Plasmid

    #220468

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    SUMO_Eu1
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSliceRep_B_miR-7.M4

    Plasmid

    #220469

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    SUMO_Eu1
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCEF-S15-FRB-Bla1

    Plasmid

    #190916

    Purpose

    Protein fragment complementation

    Depositor

    Jakob Reiser

    Article

    Ou et al Gene Ther. 2013 Jan;20(1):43-50. doi: 10

    Insert

    S15-FRB-Bla1 fusion protein
    

    Use

    Tags

    N-terminal fragment of BLA fused to c-Src and FRB…

    Expression

    Mammalian

    Mutation

    Promoter

    CEF

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSWD55

    Plasmid

    #158618

    Purpose

    Short Fibronectin FRET standard

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    Short FRET Standard
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Xylose

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSWD56

    Plasmid

    #158619

    Purpose

    Long Fibronectin FRET Standard

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    Long FRET Standard
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Xylose

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAW228

    Plasmid

    #113242

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->tAggtag

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAW227

    Plasmid

    #113241

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->Atggtag

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAW207

    Plasmid

    #113247

    Purpose

    E. coli expression vector for IHFa K20A + IHFb

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    IHFa K20A (ihfA E. coli)
    IHFb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K20A

    Promoter

    T7

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAW205

    Plasmid

    #113245

    Purpose

    E. coli expression vector for IHFa D14A + IHFb

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    IHFa D14A (ihfA E. coli)
    IHFb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D14A

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAW217

    Plasmid

    #113239

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtaC

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAW215

    Plasmid

    #113237

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggAag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge