We narrowed to 9,317 results for: bli

1. pAAV-CMV-FLEX-SaCas9-U6-sgNts

   Plasmid

   #159909

   Purpose

   Mutagenesis of Nts

   Depositor

   Larry Zweifel

   Insert

   Nts (Nts Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNHA_OROV_Gc

   Plasmid

   #208936

   Purpose

   Expresses N-terminally HA-tagged Oropouche orthobunyavirus Gc protein from CMV promoter in mammalian cells

   Depositor

   Gerald Barry

   Insert

   Gc
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted 1579 bp from 5' end, deleted 277 bp …

   Promoter

   CMV

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS_flGAD

   Plasmid

   #87031

   Purpose

   Expresses GAD (Glutamic Acid Decarboxylase) in Bacteria

   Depositor

   Janice Blum

   Insert

   flGAD (GAD2 Human)
   

   Expression

   Bacterial

   Promoter

   Lac-Z

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAP3K6-TAP

   Plasmid

   #69727

   Purpose

   Express tandem tagged MAP3K6 in cells

   Depositor

   Daniel Liebler

   Insert

   Apoptosis signal-regulating kinase 2 (MAP3K6 Human)
   

   Tags

   HA-V5 tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. XLone-eGFP NLS (no drug resistant gene)

   Plasmid

   #140032

   Purpose

   Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-GST-hSYK

   Plasmid

   #211892

   Purpose

   Express human SYK in mammalian cells

   Depositor

   Haian Fu

   Insert

   SYK (SYK Human)
   

   Tags

   GST tag

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-GATA3gfp

   Plasmid

   #83819

   Purpose

   Mammalian expression of GATA3 fused to GFP

   Depositor

   Douglas Engel

   Insert

   GATA3 (Gata3 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

   Plasmid

   #121174

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-jGCamp7b-P2A-GSG-mRuby3

   Plasmid

   #130900

   Purpose

   bicistronic plasmid for dual-color neuronal calcium and morphology imaging

   Depositor

   Simon Wiegert

   Insert

   jGCaMP7b-P2A-mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

    Plasmid

    #159920

    Purpose

    Mutagenesis of Vamp2

    Depositor

    Larry Zweifel

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYFP-NEAT1pr_v1-RT

    Plasmid

    #97087

    Purpose

    Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.

    Depositor

    Archa Fox

    Insert

    nuclear paraspeckle assembly transcript 1 (NEAT1 Human)
    

    Use

    CRISPR

    Tags

    EYFP

    Promoter

    CMV

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3XFlag-S2P

    Plasmid

    #32966

    Purpose

    For mammalian expression of human S2P with a 3x FLAG tag

    Depositor

    Ron Prywes

    Insert

    S2P (MBTPS2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

13. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid

    #121170

    Purpose

    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    ABEmax-P2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    P2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. 4LG8

    Plasmid

    #60095

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PRPF19:JMC069-A01:C226630 (PRPF19 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. MINA

    Plasmid

    #39054

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MINA (RIOX2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.3_SARS2_BA.2.75.2

    Plasmid

    #194498

    Purpose

    expressing SARS-CoV-2 BA2.75.2 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (BA.2.75.2) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    R346T, F486S, D1199N and c-terminal 18aa del on t…

    Promoter

    CMV

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #211344

    Purpose

    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection

    Depositor

    Monkol Lek

    Insert

    m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
    

    Use

    TALEN

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti.Syn(0.5).H2B-eGFP.W

    Plasmid

    #51004

    Purpose

    Lentivector that expresses H2B-eGFP from neurons-specific synapsin promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-EGFP (H2BC11 Synthetic, Human, Jellyfish)
    

    Use

    Lentiviral

    Tags

    histone2B

    Expression

    Mammalian

    Promoter

    rat synapsin

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pINDUCER20-hMYOG-3xHA (#733)

    Plasmid

    #78342

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYOG ORF with a 3xHA tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYOG (MYOG Human)
    

    Use

    Lentiviral

    Tags

    3x HA tag

    Promoter

    TRE2

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pVimentin-Spot-Tag

    Plasmid

    #117059

    Purpose

    For mammalian expression of vimentin with C-terminal Spot-Tag

    Depositor

    Felix Hartlepp

    Insert

    vimentin (VIM Human)
    

    Tags

    Spot-Tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid

    #196722

    Purpose

    Tet-inducible expression of Cas9-T2A-miRFP670nano

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Promoter

    TRE, PGK

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1 Flag-Eya2

    Plasmid

    #49264

    Purpose

    expresses Flag tagged Eya2

    Depositor

    Heide Ford

    Insert

    Eya2 (EYA2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

23. SIRT6

    Plasmid

    #41564

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    aa 3-318

    Promoter

    T7

    Available Since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

24. YWHAG

    Plasmid

    #39129

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    YWHAG (YWHAG Human)
    

    Tags

    Tev His6

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pActin5c-CD-dCas9m4-UDI-attB

    Plasmid

    #104879

    Purpose

    To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9

    Depositor

    Christopher Potter

    Insert

    CD-dCas9(m4)-UDI
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. PTPN4

    Plasmid

    #38828

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPN4 (Ptpn4 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pLEX307-CTSL-puro

    Plasmid

    #158466

    Purpose

    Constitutive lentiviral expression of human CTSL.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    CTSL (CTSL Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. FSW_SUN1_GFP

    Plasmid

    #205985

    Purpose

    Expresses hSyn1-driven, GFP-tagged SUN1 to label neuronal nuclei

    Depositor

    Ronald Hart

    Insert

    SUN1 (Sun1 Mouse)
    

    Use

    Lentiviral

    Tags

    Superfolder GFP

    Promoter

    hSyn1

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDS-Red1-N1 AMPA GluR-B(flip)

    Plasmid

    #109142

    Purpose

    The expression vector carries the red fluorescent protein tagged at the C-terminus of GluA2 subunit flip spliced form of the AMPA receptor.

    Depositor

    Stefano Vicini

    Insert

    AMPA receptor
    

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Glua2 subunit flip spliced form of the AMPA recep…

    Promoter

    CMV

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid

    #180153

    Purpose

    td-sfGFP dre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Mouse Targeting; Dre/rox

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. RAB3B (3DZ8)

    Plasmid

    #32879

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB3B (RAB3B Human)
    

    Tags

    His tag (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 18-190 only

    Available Since

    Jan. 11, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

32. pMT-TRAF6-V5-His

    Plasmid

    #63752

    Purpose

    Expresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    TRAF6 (Traf6 Fly)
    

    Tags

    V5-His

    Expression

    Insect

    Promoter

    pMT

    Available Since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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33. SRPK2

    Plasmid

    #39047

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SRPK2 (SRPK2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Internal segment 268-518 deleted; codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pZC13 (pFA6a-TEV-6xGly-V5-HphMX)

    Plasmid

    #44085

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, HphM…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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35. pDGB3_alpha1_35S-eGFP-T35S (GB4279)

    Plasmid

    #225434

    Purpose

    Transcriptional unit for constitutive expression of the enhanced GFP.

    Depositor

    Diego Orzaez

    Insert

    35S:eGFP:T35S
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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36. pEMB52 / Human_LRRK1_1_360

    Plasmid

    #40549

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK1 (LRRK1 Human)
    

    Use

    Baculovirus

    Tags

    6xHis

    Mutation

    WT

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. HaloTag-G protein gamma-12

    Plasmid

    #67043

    Purpose

    G protein gamma-12 tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-G protein gamma-12 (GNG12 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. hMYOG-3xHA-IRES-hrGFP (#324)

    Plasmid

    #78341

    Purpose

    Overexpression of human MYOG ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYOG (MYOG Human)
    

    Tags

    3x HA tag and IRES-hrGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pEN_TT CXADR-V5

    Plasmid

    #220358

    Purpose

    Gateway entry vector for an inducible V5-tagged CXADR

    Depositor

    Kevin Janes

    Insert

    CXADR (CXADR Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    V5

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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40. PAS_RvSAHS6

    Plasmid

    #215647

    Purpose

    Expresses SUMO-tagged RvSAHS6 protein from tardigrade R. varieornatus in E. coli

    Depositor

    Pamela Silver

    Insert

    SAHS6
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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