Addgene

We narrowed to 23,428 results for: Spr;

Showing: 2181 - 2200 of 23428 results

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pSMVP-Cas9N

Plasmid

#80934

Purpose

Plasmid that expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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p(bhsp68-Cas9)

Plasmid

#65959

Purpose

Tribolium basal heat shock promoter driving Cas9

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Insert

Cas9

Use

CRISPR

Tags

nuclear localization sequence

Expression

Insect

Mutation

Promoter

bhsp

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9C

Plasmid

#80939

Purpose

Plasmid that expresses Cas9C in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_CEBPB_1

Plasmid

#64036

Purpose

Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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PX458_CEBPB_2

Plasmid

#64047

Purpose

Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMA-MsgRNA-EGFP

Plasmid

#80794

Purpose

For insertion of gRNA array containing 11-30 gRNA modules

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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sg_shuttle_RFP657

Plasmid

#134968

Purpose

Lentiviral vector for sgRNA expression

Depositor

Beat Bornhauser

Article

Huang et al Hemasphere. 2020 Sep 30;4(5):e456. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6-sgRNA; EFS-RFP657

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCD227

Plasmid

#113318

Purpose

AraC-pBAD-dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Tags

Expression

Bacterial

Mutation

R93A

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos9_AmpR

Plasmid

#119152

Purpose

Encodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-L3US2-RFP-HDAC3

Plasmid

#83966

Purpose

Lentiviral CRISPR HDAC3 dual gRNA targeting vector

Depositor

Eleanor Chen

Insert

HDAC3 dual gRNAs (HDAC3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pH1v1

Plasmid

#60244

Purpose

gRNA expression by the H1 promoter

Depositor

Donald Zack

Article

Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pMZ283

Plasmid

#52224

Purpose

Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

rrk1-sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

rrk1

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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PX458_CREB1_2

Plasmid

#64940

Purpose

Expresses gRNA against human CREB1 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCR1002

Plasmid

#71820

Purpose

Express Streptococcus pyogenes Cas9

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_10xHis-MBP-TEV-SPyCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJF131B

Plasmid

#113326

Purpose

pTet-W108 scRNA.b2, pTet-RR2-sgRNA

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

W108 scRNA_MS2 and sgRNA_RR2

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pJF104B

Plasmid

#113324

Purpose

pTet-dCas9, pTet-MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Tags

Expression

Bacterial

Mutation

R93A

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMB67

Plasmid

#47947

Purpose

Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Mutation

Promoter

hsp-16.48

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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PX458_GABPA_2

Plasmid

#64255

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMB63

Plasmid

#47945

Purpose

Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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PX458_GABPA_1

Plasmid

#64254

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge