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pEF019 MBP-β-catenin-His

Plasmid

#154070

Purpose

Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CTNNB1 (CTNNB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCjeCas9

Plasmid

#172215

Purpose

Expresses Campylobacter jejuni Cas9 (CjeCas9) in bacterial cells

Depositor

John van der Oost

Publication

Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

Insert

Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system

Tags

Histag

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

Plasmid

#156463

Purpose

Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

Depositor

Andre Stiel

Publication

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

Heme Oxygenase
ReBphP-PCM

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET14-GFP30_Encap

Plasmid

#86406

Purpose

Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Publication

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 30aa CLP tag
Encapsulin

Tags

6x Histidine

Expression

Bacterial

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RGS-SENP5m

Plasmid

#18715

Depositor

Edward Yeh

Publication

Gong et al J Biol Chem. 2006 Jun 9. 281(23):15869-7

Insert

SENP5m (SENP5 Human)

Tags

RGS

Expression

Mammalian

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRetroSuper-GFP TIF1gamma

Plasmid

#15721

Depositor

Joan Massague

Publication

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma shRNA (TRIM33 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pNP1723

Plasmid

#222727

Purpose

sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

Depositor

Patrick Hsu

Publication

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

sfGFP

Expression

Bacterial

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pES001 MBP-GSK3β-HA-His

Plasmid

#154010

Purpose

Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GSK3B (GSK3B Human)

Tags

HA tag, His6, and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hUtx-IRES-Hyg

Plasmid

#122374

Purpose

Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hUtx-IRES-Hyg-pA

Use

Piggybac

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF-DUET Cas1 Cas2

Plasmid

#80088

Purpose

Encodes Cas1 and Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 E. coli (K12))
Cas2

Expression

Bacterial

Promoter

T7

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP713

Plasmid

#45465

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MyrPalm-CKAR-T/A

Plasmid

#14863

Depositor

Alexandra Newton

Publication

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Mutation

T/A

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk2b2

Plasmid

#13757

Depositor

Roger Davis

Publication

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b2 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pTV-Oct4-TK-HMS

Plasmid

#62351

Purpose

Mouse Oct4 targeting vector

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

TK-Hyg-24xMS2

Use

Mouse Targeting

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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HO-Poly-HO

Plasmid

#51661

Purpose

yeast plasmid for integration of target sequence at HO locus

Depositor

David Stillman

Publication

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBEST-p15A-PLlacO1-deGFP-T500

Plasmid

#45394

Depositor

Vincent Noireaux

Publication

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Expression

Bacterial

Promoter

PL-lacO1

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGL3-Control-miR-17-5p-Control

Plasmid

#21167

Depositor

Joshua Mendell

Publication

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

miR 17-5p Sensor (MIR17 Human)

Expression

Mammalian

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBMTBX-2

Plasmid

#26073

Depositor

Ryan Gill

Publication

Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGL3-p27 T187A Luc

Plasmid

#23048

Depositor

William Kaelin

Publication

Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

Insert

p27 (CDKN1B Human)

Tags

Luciferase

Expression

Mammalian

Mutation

T187A: mutation at codon 187 changing from T (ACG…

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PBGFAP-eGFP

Plasmid

#40975

Depositor

Joseph Loturco

Publication

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

Mouse GFAP promoter fragment

Expression

Mammalian

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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