We narrowed to 7,248 results for: Cit

1. pD2sE_SC10

   Plasmid

   #219716

   Purpose

   DV2 stable E dimer with fusion loop mutation

   Depositor

   Brian Kuhlman

   Article

   Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

   Insert

   DV2 sE SC10
   

   Use

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A259W, T262R, F279W, T280P

   Promoter

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEF073 MBP-Axin-His

   Plasmid

   #154011

   Purpose

   Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   Axin1 (AXIN1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDestTol2-actinb-kcnk9-IRES-EGFP

   Plasmid

   #164949

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnk9 (kcnk9 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   actinb (actb2) promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 416Gal-FUS-R521C-YFP

   Plasmid

   #29622

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   R521C

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pLEX307-SARS-CoV-3CL

   Plasmid

   #160280

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLC-CUL4A K705R-P2A-Puro

   Plasmid

   #124305

   Purpose

   Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   CUL4A (CUL4A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Lysine 705 to Arginine

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FCK-Chronos-GFP

   Plasmid

   #80902

   Purpose

   Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA 3.2-FUS-1-413aa-V5

   Plasmid

   #29612

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS-1-413aa (FUS Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Contains FUS aa# 1-413

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

9. 3I91

   Plasmid

   #62514

   Purpose

   Bacterial expression plasmid for use in structural determination.

   Depositor

   Cheryl Arrowsmith

   Article

   Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

   Insert

   CBX8 (CBX8 Human)
   

   Use

   Tags

   6xHis and TEV cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pcDNA 3.2-FUS-1-269aa-V5

    Plasmid

    #29610

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-269aa (FUS Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Contains FUS aa# 1-269

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. 416Gal-FUS-R521G-YFP

    Plasmid

    #29623

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R521G

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pLV-shEsr2

    Plasmid

    #120722

    Purpose

    Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

    Depositor

    Amy Lasek

    Article

    Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

    Insert

    Esr2 (Esr2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGAL4-DBD-CEBPa-WT

    Plasmid

    #215601

    Purpose

    For overexpression of Gal4-DBD CEBPa WT

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    CEBPa (CEBPA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

    Plasmid

    #160571

    Purpose

    Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNAscaffold 2.1 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA 3.2-FUS-1-373aa-V5

    Plasmid

    #29611

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-373aa (FUS Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Contains FUS aa# 1-373

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pHH0103_LYN-1/1

    Plasmid

    #91536

    Purpose

    Protein expression and purification of human SH3 domain construct LYN-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    LYN-1/1 (LYN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LV_EF1a_CDK1-P2A-Hygro_Barcode

    Plasmid

    #170221

    Purpose

    Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTE4495

    Plasmid

    #80338

    Purpose

    mammalian expression MbCpf1 nuclease and Mb crRNA

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    MbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 416GPD-FUS-YFP

    Plasmid

    #29594

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pTE4938

    Plasmid

    #107527

    Purpose

    Expresses Fn crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPB-TetON-mEGFP-NGN2_WT

    Plasmid

    #215610

    Purpose

    For integration of NGN2 WT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)

    Plasmid

    #160622

    Purpose

    TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s_MS2:EDLL_Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHH0103_ARHGAP32-1/1

    Plasmid

    #91312

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP32-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP32-1/1 (ARHGAP32 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28-p53 (1-90) (72P)

    Plasmid

    #62080

    Purpose

    Expression of human p53 (1-90) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD/PP residues 1-90; 72P

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pMG058 MBP-GS 634-666-His

    Plasmid

    #154077

    Purpose

    Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GYS1 (GYS1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLEX307-HCoV-NL63-3CL-C144A

    Plasmid

    #160287

    Purpose

    Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C144A

    Promoter

    EF1a

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_ABL1-1/1

    Plasmid

    #91521

    Purpose

    Protein expression and purification of human SH3 domain construct ABL1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ABL1-1/1 (ABL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_ASAP2-1/1

    Plasmid

    #91504

    Purpose

    Protein expression and purification of human SH3 domain construct ASAP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ASAP2-1/1 (ASAP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103_MAP3K9-1/2

    Plasmid

    #91313

    Purpose

    Protein expression and purification of human SH3 domain construct MAP3K9-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MAP3K9-1/2 (MAP3K9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge