-
Plasmid#221268PurposeExpression of IRSp53 pLxIS (247-375) in mammalian cells by retroviral transductionDepositorInsertIRSp53 pLxIS (247-375) (Baiap2 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-IRSp53(pLxIS) S269A
Plasmid#221269PurposeExpression of IRSp53 pLxIS mutant in mammalian cells by retroviral transductionDepositorInsertIRSp53 pLxIS (Baiap2 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationS269APromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-GMIP(pLxIS) S31A
Plasmid#221271PurposeExpression of GMIP pLxIS mutant in mammalian cells by retroviral transductionDepositorInsertGMIP pLxIS (Gmip Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationS31APromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-FAM13A
Plasmid#185550PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting FAM13ADepositorInsertFAM13A gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-Doc2β C2AB (G361C)
Plasmid#213631PurposeBacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)DepositorInsertDoc2β C2AB (G361C) (Doc2b Rat)
UseTagsSUMOExpressionBacterialMutationG361C, all original cysteines mutated to alaninesPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0108
Plasmid#143011PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTRERF1 (TRERF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0106
Plasmid#142536PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTRERF1 (TRERF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-P66KP71Q-GB1_AD
Plasmid#148516PurposeBacterial Expression of DmThor_50-83-P66KP71QDepositorInsertDmThor_50-83-P66KP71Q (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HseIF4G_571-676-GB1_AG
Plasmid#148745PurposeBacterial Expression of HseIF4G_571-676DepositorInsertHseIF4G_571-676 (EIF4G1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HseIF4G_592-653_AG
Plasmid#148746PurposeBacterial Expression of HseIF4G_592-653DepositorInsertHseIF4G_592-653 (EIF4G1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676-GB1_AD
Plasmid#148512PurposeBacterial Expression of DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676DepositorInsertDmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-DSP
Plasmid#185549PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting DSPDepositorInsertDSP gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G1_615-654_AD
Plasmid#148518PurposeMammalian Expression of HseIF4G1_615-654DepositorInsertHseIF4G1_615-654 (EIF4G1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G1_615-654-L640AV646A_AD
Plasmid#148519PurposeMammalian Expression of HseIF4G1_615-654-L640AV646ADepositorInsertHseIF4G1_615-654-L640AV646A (EIF4G1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD
Plasmid#148523PurposeMammalian Expression of HseIF4G1_615-654-LIVVtoAAAADepositorInsertHseIF4G1_615-654-LIVVtoAAAA (EIF4G1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G_615-654-Y619AL624AL625A_AD
Plasmid#148524PurposeMammalian Expression of HseIF4G1_615-654-Y619AL624AL625ADepositorInsertHseIF4G1_615-654-Y619AL624AL625A (EIF4G1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-HseIF4E_AF
Plasmid#148669PurposeBacterial Expression of HseIF4EDepositorInsertHseIF4E (EIF4E Human)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence of i…PromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
prCOrev
Plasmid#153961PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCO
Plasmid#153960PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g3 (BB24)
Plasmid#139459PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only