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pHCMV_FLUBV_HA

Plasmid

#207274

Purpose

Mammalian expression of Influenza B virus HA protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

FLUBV_HA (HA Influenza B virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV-SS-antiEEscFv-TM

Plasmid

#198304

Purpose

Displays anti-EE scFv from the surface of mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pHCMV-SS-antiEEscFv-TM

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a LEU2

Plasmid

#61923

Purpose

Expression of K. lactis LEU2 cloned into pFA6a

Depositor

Jon Houseley, David Tollervey

Article

Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

Insert

LEU2

Use

Yeast genomic targeting

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pVM013

Plasmid

#64768

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-GFP tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV-SS-antiSunTag-TM

Plasmid

#198306

Purpose

Displays anti-SunTag scFv from the surface of mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pHCMV-SS-antiSunTag-TM

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-tglB

Plasmid

#134676

Purpose

Expresses His6-TglBin E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglB

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAureus-TnCAM

Plasmid

#199807

Purpose

vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

Depositor

Steve Salipante

Article

Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

Insert

transposon cassette

Expression

Bacterial

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETa_ mEos4b

Plasmid

#51073

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

Depositor

Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4b

Expression

Bacterial

Promoter

T7

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pMSSM151: pU6-NlovFz2_omegaRNA-entry

Plasmid

#205263

Purpose

Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

Plasmid

#55219

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-surfaceHA-IRES-Puro

Plasmid

#207321

Purpose

Lentiviral expression of synthetic HA surface receptor in Mammalian cells

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HAtag-Myctag-PDGFRB-TM-IRES-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha_short (EFS)

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMD2.G-H8A/K47Q

Plasmid

#207322

Purpose

VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

VSV-G (VSIVgp4 Vesicular Stomatitis Virus)

Expression

Mammalian

Mutation

H8A/K47Q

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-FLAG

Plasmid

#198297

Purpose

Produces PVCs retargeted with FLAG tag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-FLAG

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

Plasmid

#205268

Purpose

Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L DD

Plasmid

#27001

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Mutation

C962A

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pHCMV_ALV_Env

Plasmid

#207325

Purpose

Expression of env polyprotein from Avian leukosis virus (ALV)

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

ALV_Env (env Avian leukosis virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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GST-mCh-FYVE

Plasmid

#192289

Purpose

Expresses GST- and mCherry-tagged HRS FYVE in e Coli

Depositor

James Hurley

Article

Jensen et al Sci Adv. 2022 Dec 14;8(50):eadd1436. doi

Insert

HRS FYVE domain

Tags

GST-TEV-mCherry

Expression

Bacterial

Promoter

tac

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_POWV_PrME

Plasmid

#207299

Purpose

Mammalian expression of Tick-borne powassan virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

POWV_PrME (POLY Tick-borne powassan virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-CD4DARPin

Plasmid

#198294

Purpose

Produces PVCs retargeted with anti-CD4 DARPin D27.2

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-CD4DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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DT037A

Plasmid

#159398

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23101 (strong) promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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