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pSAM2_mCherry_SRF

Plasmid

#72695

Purpose

Doxycyline regulated expression of SRF in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

serum response factor (SRF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp1

Plasmid

#72687

Purpose

Doxycyline regulated expression of Mesp1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 1 (MESP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-CD79A-YFP

Plasmid

#187004

Purpose

Expression of human CD79A as YFP fusion protein

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

CD79A (CD79A Human)

Use

Lentiviral

Tags

YFP

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Irx4

Plasmid

#72692

Purpose

Doxycyline regulated expression of Irx4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Irx 4 (IRX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Isl1

Plasmid

#72696

Purpose

Doxycyline regulated expression of Isl1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Islet1 (Isl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-CD79A-RFP

Plasmid

#187003

Purpose

Expression of human CD79A as RFP fusion protein

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

CD79A (CD79A Human)

Use

Lentiviral

Tags

tagRFP

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-BATF

Plasmid

#178451

Purpose

doxycycline-inducible expression of Human BATF in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

BATF (BATF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

Plasmid

#179843

Purpose

Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

Luciferase and Synthetic Biology

Tags

eGFP and luciferase

Expression

Mammalian

Promoter

TRE3G

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata3

Plasmid

#128824

Purpose

dox-dependent expression of mouse Gata3

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Gata3 (Gata3 Mouse)

Use

Lentiviral

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Eomes

Plasmid

#128825

Purpose

dox-dependent expression of mouse Eomes

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Eomes (Eomes Mouse)

Use

Lentiviral

Mutation

Shorter isoform

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK3 ct in pcDNA3.1

Plasmid

#64213

Purpose

mCherry fused between Cry2 and GRK3 ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK3 ct (GRK3 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-hs-HRAS

Plasmid

#52730

Purpose

retroviral expression of human HRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HRAS (HRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

Plasmid

#128835

Purpose

Knock-in of TagBFP into mouse Esrrb locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tagBFP into Esrrb locus (Esrrb Mouse)

Use

Mouse Targeting

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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G30, Cdc20-mCherry-AID tagging

Plasmid

#130270

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Article

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(S345D)-2A-mCherry-puro

Plasmid

#231975

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce phosphomimetic MLKL S345D necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL S345D construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Mutation

MLKL S345D

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(1-180)-2A-mCherry-puro

Plasmid

#231976

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro

Plasmid

#231972

Purpose

Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)

Use

Lentiviral

Mutation

WT

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_c-MYC

Plasmid

#26818

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-c-MYC-3'UTR (MYC Human)

Expression

Mammalian

Mutation

Sequence begins from second ATG; Synthetic UTR se…

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-DmrB-Casp8-2A-mCherry-puro

Plasmid

#231971

Purpose

Tet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

Casp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)

Use

Lentiviral

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AICSDP-23:TJP1-mEGFP

Plasmid

#87429

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

TJP1 Homology Arms with mEGFP-linker (TJP1 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

March 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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