We narrowed to 5,111 results for: codon optimized

1. His-Tev-UbE1

   Plasmid

   #186804

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-UbE1 (UBA1 Human)
   

   Tags

   His6-Tev

   Expression

   Bacterial

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pN7HELIX

   Plasmid

   #190662

   Purpose

   Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
   

   Expression

   Mammalian

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   CMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSN075

   Plasmid

   #166732

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-NLS-Mxi1
   

   Use

   CRISPR

   Tags

   Mxi1 and SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDB179-DepoPETase

   Plasmid

   #218702

   Purpose

   Encodes codon optimized DepoPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   DepoPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDe-CAS9

   Plasmid

   #61433

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   PcUBI4-2

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDB179-ThermoPETase

   Plasmid

   #218698

   Purpose

   Encodes codon optimized ThermoPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   ThermoPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mt POLRMT

   Plasmid

   #205059

   Purpose

   POLRMT protein expression

   Depositor

   Smita Patel

   Article

   Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

   Insert

   POLRMT (POLRMT Human)
   

   Expression

   Bacterial

   Mutation

   Codon optimized

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Promoter

   2x35S and StUbi-3P

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCMV_EEE_E1E2

   Plasmid

   #207272

   Purpose

   Mammalian expression of Eastern equine encephalitis virus E1E2 protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   EEE_E1E2 (EEEVgp3 Eastern equine encephalitis virus)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDHt/sk-PE

    Plasmid

    #92126

    Purpose

    Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei

    Depositor

    Zhihua Zhou

    Article

    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

    Insert

    Cas9-eGFP
    

    Use

    CRISPR; Trichoderma reesei expression

    Tags

    EGFP

    Promoter

    Ppdc

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDD268

    Plasmid

    #132523

    Purpose

    mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein, Daniel Dickinson

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mNG-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mNG

    Expression

    Worm

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-memRFP

    Plasmid

    #224564

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    Unspecified

    Tags

    Membrane localization signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPomyces-SaCas9

    Plasmid

    #129553

    Purpose

    Contains codon-optimized SaCas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    SaCas9
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-Tev-UbE2D3

    Plasmid

    #186805

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-UbE2D3 (UBE2D3 Human)
    

    Tags

    His6-Tev

    Expression

    Bacterial

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPomyces-FnCpf1

    Plasmid

    #129554

    Purpose

    Contains codon-optimized FnCpf1 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    FnCpf1
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDB179-BhrPETase

    Plasmid

    #218692

    Purpose

    Encodes codon optimized BhrPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    BhrPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSET-dCas9

    Plasmid

    #110183

    Purpose

    Expression of the dCas9 gene in Streptomyces

    Depositor

    Yinhua Lu

    Article

    Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

    Insert

    dCas9
    

    Use

    CRISPR; I

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    ermE*p

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPomyces-Sth1Cas9

    Plasmid

    #129552

    Purpose

    Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    Sth1Cas9
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDB179-CaPETaseM9

    Plasmid

    #218707

    Purpose

    Encodes codon optimized CaPETaseM9 enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    CaPETaseM9
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYZ960

    Plasmid

    #187972

    Purpose

    TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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