We narrowed to 6,558 results for: sas

1. pLKO_HA.NWS.FLAG_NGFR

   Plasmid

   #158249

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.NWS.FLAG

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p029 End-1::EnR

   Plasmid

   #11029

   Depositor

   Ramesh Shivdasani

   Article

   Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

   Insert

   End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)
   

   Use

   Xenopus, mammalian, avian, and zebrafish

   Mutation

   Dominant inhibitory GATA-binding protein End1::En…

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-sACE2v3-sfGFP

   Plasmid

   #145174

   Purpose

   Mammalian expression plasmid for sACE2-sfGFP high affinity variant 3

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Linker GSGGSGSGG and superfolder GFP

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-sACE2v6-Fc(IgG1)

   Plasmid

   #145169

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 6

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO_HA.AU1.VSVg_NGFR

   Plasmid

   #158232

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.AU1.VSVg

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-sACE2v6-Fc(IgA1)

   Plasmid

   #145161

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 6

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO_NWS.AU1.VSVg_NGFR

   Plasmid

   #158239

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   NWS.AU1.VSVg

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

   Plasmid

   #199263

   Purpose

   Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

   Depositor

   Erik Sontheimer

   Article

   Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

   Insert

   NmeABE8e
   

   Use

   AAV and CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. NC4 pCDNA3.1 VENUS WTX (1-804)

   Plasmid

   #36959

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX (AMER1 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pD649-HAsp-VCAM1-COMP5AP-AviTag-9xHis

    Plasmid

    #157506

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    VCAM1 (VCAM1 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mOrange-P2A-hKvb1

    Plasmid

    #154084

    Purpose

    Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter

    Depositor

    Michael Hoppa

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

    Insert

    mOrange-P2A-human Kvb1 (KCNAB1 Human, Synthetic)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human synapsin 1

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-VSTM2L-COMP5AP-AviTag-9xHis

    Plasmid

    #157146

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    VSTM2L (VSTM2L Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-f1(+)-TOMM20-mEGFP

    Plasmid

    #115911

    Purpose

    donor to insert EGFP at the TOMM20 locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    TOMM20-mEGFP (TOMM20 Human)
    

    Use

    Donor template

    Tags

    EGFP

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDF1-MCS2-EF1-Puro-RGS7

    Plasmid

    #113728

    Purpose

    Express RGS7 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD649-HAsp-TNFRSF6B-COMP5AP-AviTag-9xHis

    Plasmid

    #157277

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TNFRSF6B (TNFRSF6B Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pD649-HAsp-SEMA3G-COMP5AP-AviTag-9xHis

    Plasmid

    #157539

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3G (SEMA3G Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-sACE2v3-Fc(IgG1)

    Plasmid

    #145166

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 3

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-sACE2v5-sfGFP

    Plasmid

    #145176

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 5

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-sACE2v4-sfGFP

    Plasmid

    #145175

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 4

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

    Plasmid

    #86195

    Purpose

    U6 based expression of N meningitidis sgRNA

    Depositor

    Scot Wolfe

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    nmCas9 sgRNA cloning cassete
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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