We narrowed to 4,785 results for: Mos
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Plasmid#188892PurposettTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 markerDepositorInsertPmex-5::Cas9, Cbr-unc-119
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgTS40
Plasmid#169630PurposepX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.DepositorInsertU6-driven gRNA expression and PCAG-driven SpCas9 expression
UseTagsExpressionMammalianMutationPromoterU6 / PCAGAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lmajor_gp63_10_0460_P460G_D463N_S465A
Plasmid#171645PurposeExpression of L. major glcyoprotein-63 (P460G_D463N_S465A) with substrate binding site mutations from chromosome 10 in mammalian cellsDepositorInsertLmjF.10.0460 P460G D463N S465A
UseTagsMyc-HisExpressionMammalianMutationP460G; D463N; S465APromoterAvailable sinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT.334
Plasmid#119556PurposeLevel T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/CTP-fluoA
Plasmid#117990PurposeChloroplast-targeted expression of CURT_fluoA controlled by the CaMV 35S promoterDepositorInsertCTP-CURT_fluoA
UseSynthetic Biology; Plant/bacteria binary vectorTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/CTP-fluoB
Plasmid#117991PurposeChloroplast-targeted expression of CURT_fluoB controlled by the CaMV 35S promoterDepositorInsertCTP-CURT_fluoB
UseSynthetic Biology; Plant/bacteria binary vectorTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/CTP-fluoE
Plasmid#117992PurposeChloroplast-targeted expression of CURT_fluoE controlled by the CaMV 35S promoterDepositorInsertCTP-CURT_fluoE
UseSynthetic Biology; Plant/bacteria binary vectorTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/CURT_flouB
Plasmid#117995PurposeExpression of CURT_flouB controlled by the CaMV 35S promoterDepositorInsertCURT_flouB
UseYeast/bacteria binary vectorTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB6637
Plasmid#106164PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pL
Plasmid#196286Purpose35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)DepositorInsertFull length TSWV L antigenome encoding codon-optimized L (RdRp)
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOm
Plasmid#189930PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMZ7
Plasmid#223180PurposepORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.DepositorTypeEmpty backboneUseChromosomal integrationTagsExpressionMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP (F64L, S65T, V163A)-TRP1
Plasmid#53184Purposechromosomal tagging with stable GFPDepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A)ExpressionMutationPromoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2-UTX-F
Plasmid#17438PurposeExpression of UTX in mammalian cellsDepositorInsertUTX (KDM6A Human)
UseTags6xMyc and FlagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- C11orf83-GFP
Plasmid#65844PurposeMammalian expression of C terminally GFP-tagged C11orf83/UQCC3DepositorInsertUQCC3 WT (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationWTPromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJWV102-PL-dCas9
Plasmid#85588PurposeIntegrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9spDepositorInsertdCas9sp
UseCRISPRTagsExpressionBacterialMutationPromoterPLspAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-3XNLS
Plasmid#209392PurposeTransiently expressing TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL17
Plasmid#106483PurposepDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosomeDepositorInsertsgam
bet
exo
lacI
rhaR
rhaS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD4-Nter-APEX2
Plasmid#112868PurposeBacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner methodDepositorInsertAPEX2
UseTagsExpressionMutationPromoterAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP_cSuMMVp
Plasmid#119182PurposeExpresses C-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FK-506 binding protein (FKBP)DepositorInsertsplit cSuMMVp in fusion with FKBP
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSuMMVp
Plasmid#118970PurposeExpresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSuMMVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGG-cTP-TurboID-GFP
Plasmid#209403PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in plantaDepositorInsertPM-3XHA-TurbOID
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-GFP-TurboID-3XNLS
Plasmid#209400PurposeTransiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-KSENB-Nup133
Plasmid#87340PurposeTo express human Nup133 in mammalian cellsDepositorInsertNUP133 (NUP133 Human)
UseTagsEGFPExpressionMammalianMutationdeletion of Nup133 AA1-7PromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB114
Plasmid#68568PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJW31
Plasmid#136423PurposeContains genes of Type I-C CRISPR-Cas system for inducible expression and chromosomal integration at attTn7DepositorInsertsCas5
Cas8
Cas7
Cas3
Cas3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB405
Plasmid#68670PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB402
Plasmid#68667PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-gRNA-puro
Plasmid#180426PurposeExpresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.DepositorInsertgRNAscr
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB435
Plasmid#68700PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGG-GFP-PM-TurboID
Plasmid#209402PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in plantaDepositorInsertcTP-3XHA-TurboID
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM4937 (pCV0049)
Plasmid#120082PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGIP*35
Plasmid#26776PurposeEnables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targetingDepositorInsertGFP*35
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSbMVp
Plasmid#118969PurposeExpresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSbMVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
UseTagsExpressionWormMutationPromotereft-3Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP_cSbMVp
Plasmid#118968PurposeExpresses C-terminus of soybean mosaic virus protease (SbMVp) fused to the FK-506 binding protein (FKBP)DepositorInsertsplit cSbMVp in fusion with FKBP
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLS791
Plasmid#188889PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFPDepositorInsertfloxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAH49 - [PVQp | mScarlet | tbb-2 UTR]
Plasmid#200325PurposemScarlet BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[PVQp | wrmscarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003755, nlp-17Available sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only