We narrowed to 59,329 results for: cloning

1. pCAGEN-DV-IRES-myr-BFP (JDW 491)

   Plasmid

   #229834

   Purpose

   A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR_P4-P1R:PER36modp

   Plasmid

   #128431

   Purpose

   Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).

   Depositor

   George Haughn

   Article

   McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

   Insert

   PEROXISADE36 (AT3G50990 Mustard Weed)
   

   Use

   Gateway promoter entry clone

   Mutation

   Premature stop codons introduced into the Xero1 (…

   Promoter

   PEROXISADE36

   Available Since

   Oct. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pIRES-EGFP-puro

   Plasmid

   #45567

   Depositor

   Michael McVoy

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   IRES-EGFP-Puro

   Expression

   Mammalian

   Promoter

   Phcmv (strong human cytomegalovirus immediate ear…

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-MHL

   Plasmid

   #26096

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLVX TRE 3G BFP

   Plasmid

   #128070

   Purpose

   Lentiviral expression of Tet inducible TagBFP

   Depositor

   Oskar Laur

   Insert

   TagBFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   no

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIVTRup

   Plasmid

   #101362

   Purpose

   Highly efficient in vitro transcription. Empty backbone.

   Depositor

   Ángel Raya

   Insert

   5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
   

   Use

   For cloning a gene for in vitro transcription pur…

   Promoter

   T7

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCVhyg-DEST

   Plasmid

   #119747

   Purpose

   Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Hygromycin resistance)

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. plix403-V5-N-mEGFP

   Plasmid

   #223957

   Purpose

   Gateway cloning of genes of interest with an N-terminal V5-mEGFP fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5-mEGFP

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. plix403-V5-N-mCherry

   Plasmid

   #223956

   Purpose

   Gateway cloning of genes of interest with an N-terminal V5-mCherry fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5-mCherry

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-IL-2 signal

    Plasmid

    #128028

    Purpose

    Mammalian expression vector with N terminal IL-2 signal peptide

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    IL-2 signal peptide

    Expression

    Mammalian

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-EF1-IRES plum

    Plasmid

    #128072

    Purpose

    gfp was exchanged to plum

    Depositor

    Oskar Laur

    Insert

    plum
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    no

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLOC CMV-IRES-Dsred-blasticidin

    Plasmid

    #129428

    Purpose

    expression of Dsred-blasticidin under CMVpromoter -IRES

    Depositor

    Oskar Laur

    Insert

    Dsred-blasticidin
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. plix403-V5-N-mECFP

    Plasmid

    #223954

    Purpose

    Gateway cloning of genes of interest with an N-terminal V5-mECFP fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Karla Feijs, Roko Žaja

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5-mECFP

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC-Dest-BsaI

    Plasmid

    #200550

    Purpose

    Mini circle destination vector with BsaI sites for Golden Gate Assembly

    Depositor

    Junsu Kang

    Type

    Empty backbone

    Use

    Minicircle cloning vector

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC-L3L2

    Plasmid

    #114019

    Purpose

    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

    Depositor

    Christopher Grefen

    Insert

    ccdB gene, Chloramphenicol resistance gene
    

    Use

    Gateway

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC-L1L4

    Plasmid

    #114018

    Purpose

    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

    Depositor

    Christopher Grefen

    Insert

    ccdB gene, Chloramphenicol resistance gene
    

    Use

    Gateway

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MZB117

    Plasmid

    #228481

    Purpose

    "Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Tags

    GST (glutathione S-transferase)

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRC-LIC

    Plasmid

    #62343

    Purpose

    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    tac promoter from pKK233-2

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUW-mTie1

    Plasmid

    #119234

    Purpose

    Plasmid encodes full-length murine Tie1 gene cloned in lentiviral vector.

    Depositor

    Kari Alitalo

    Insert

    Tie1 (Tie1 Mouse)
    

    Use

    Lentiviral

    Promoter

    P hUbC

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC-L1L2

    Plasmid

    #114017

    Purpose

    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

    Depositor

    Christopher Grefen

    Insert

    ccdB gene, Chloramphenicol resistance gene
    

    Use

    Gateway

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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