We narrowed to 5,304 results for: pcDNA3.1(+)

1. pcDNA3.1_HA-p115-TEV-cleaved #957#382

   Plasmid

   #42107

   Depositor

   Ayano Satoh

   Article

   Koreishi et al Antibody Technology Journal. 2011;1:5-11

   Insert

   p115
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted 886 to end and added ENLYFQ

   Promoter

   CMV

   Available Since

   Feb. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

   Plasmid

   #138198

   Purpose

   FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and cpV[E172]

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES

   Plasmid

   #69909

   Purpose

   Expresses a GFP circular RNA containing the sense EMCV IRES in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

   Plasmid

   #79620

   Purpose

   Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1 and UGI

   Expression

   Mammalian

   Mutation

   D10A for nickase Cas9

   Promoter

   pCMV

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

   Plasmid

   #202606

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-P2A-mCherry-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)

   Plasmid

   #60606

   Purpose

   Mammalian Display vector

   Depositor

   Wayne Marasco

   Article

   Taube et al PLoS One. 2008 Sep 11;3(9):e3181. doi: 1

   Insert

   PS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
   

   Use

   Tags

   C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. (344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

   Plasmid

   #168097

   Purpose

   HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nGFP-(EAAAK)4-OGA(544-706)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

   Plasmid

   #168102

   Purpose

   HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nEPEA-(EAAAK)3-OGA(544-706)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   OGA truncated to contain residues 544-706

   Promoter

   T7/CMV

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201819

   Purpose

   A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pOTTC1751

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) CircRNA Mini Vector-ZKSCAN1 nt 548-1047

    Plasmid

    #60649

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Antisense IRES

    Plasmid

    #69910

    Purpose

    Expresses a GFP circular RNA containing the antisense EMCV IRES in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1, GFP (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. (424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)

    Plasmid

    #168191

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutation

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nLag6-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid

    #73209

    Purpose

    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Human, Synthetic)
    

    Use

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1417 (No intron)

    Plasmid

    #69898

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F169L in ZKSCAN1

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

    Plasmid

    #171375

    Purpose

    For the mammalian expression of rat Gαi3 internally tagged with YFP

    Depositor

    Mikel Garcia-Marcos

    Article

    Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

    Insert

    Gai3-YFP
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

    Plasmid

    #58275

    Purpose

    mammalian expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

17. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

    Plasmid

    #168099

    Purpose

    HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nUbc-OGA(544-706)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-FLAG-Dicer-Y926F/R927A (3'-pocket mutant)

    Plasmid

    #51369

    Depositor

    Narry Kim

    Article

    Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

    Insert

    Dicer (DICER1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    3' pocket mutant (Y926F, R927A)

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-sfVenus-iGluSnFR

    Plasmid

    #187097

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-sfVenus-iGluSnFR
    

    Use

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-iRFP-P2A-Post-SPHERE-sfVenus-iGluSnFR

    Plasmid

    #187098

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-sfVenus-iGluSnFR
    

    Use

    Tags

    iRFP670-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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