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We narrowed to 25,004 results for: CHI

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Showing: 5651 - 5700 of 25004 results

  2. pCAGGS-SMS1-TEV-Twin-Strep

    Plasmid
    #202528
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS1 (SGMS1 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202528-plasmid-map-sequence-id-406371

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  13. pCAGGS-PHFF

    Plasmid
    #100280
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100280-plasmid-map-sequence-id-194335

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  17. pcDNA3.1-nAChr-Alpha6-mCherry

    Plasmid
    #50480
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    mCherry fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. SSH-pSUMO-α-COPI-WD40

    Plasmid
    #208341
    Purpose
    Bacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI (1-327) with substitutions (Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys)
    Tags
    His-tag, SUMO-tag, and Strep-tag
    Expression
    Bacterial
    Mutation
    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Ph…
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208341-plasmid-map-sequence-id-436201

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  21. USPL1cat-C236S-pEYFP_C1

    Plasmid
    #85767
    Purpose
    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236S
    Depositor
    Frauke Melchior
    Article
    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10
    Insert
    USPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    catalytic domain (aa 212-514), catalytic inactiv…
    Promoter
    CMV
    Available Since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85767-plasmid-map-sequence-id-169512

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  23. pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant

    Plasmid
    #72922
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Gateway Cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Promoter
    TRE tight
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72922-plasmid-map-sequence-id-138771

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  45. Coexpressed Free NES

    Plasmid
    #182482
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182482-plasmid-map-sequence-id-360445

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  47. EcNGsC-DelFSH(307-309)-pKK223

    Plasmid
    #131377
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.
    Depositor
    Martin Horvath
    Article
    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021
    Insert
    EcNGsC MutY chimera DelFSH(307-309)
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are from…
    Promoter
    tac
    Available Since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131377-plasmid-map-sequence-id-259231

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  50. pEN_TT 3XFLAG-S37A-GATA6-3XAU1

    Plasmid
    #72613
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Gateway Cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Mutation
    Changed serine37 to alanine
    Promoter
    TRE tight promoter
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72613-plasmid-map-sequence-id-138777

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Showing: 5651 - 5700 of 25004 results