We narrowed to 9,406 results for: Coli
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Plasmid#67012PurposeExpresses malE-ELP[AV-60] in E. coliDepositorInsertmalE-ELP[AV-60]
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b_pScaC-SNAP_6xHis
Plasmid#228835PurposeE. coli expression of the SNAP-tagged ScaC passenger domainDepositorInsertScaC
UseTagsSNAPtag-TEV-6xHisExpressionBacterialMutationaa 33-223PromoterAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGTetRnls
Plasmid#26599DepositorAvailable SinceNov. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1_CylLS_CylM
Plasmid#208760PurposeExpresses modified cytolysin S (mCylLS) in E. coliDepositorInsertsCylLS
CylLM
UseTagsHisx6 TagExpressionBacterialMutationPromoterT7Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
MK1283
Plasmid#71428PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …PromoterAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crGE
Plasmid#172927PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsHisExpressionBacterialMutationPromoterT7Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PtxS1-c003
Plasmid#173076PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPtxS1
UseTagsExpressionBacterialMutationD35-I221PromoterAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-ELP[V-60]
Plasmid#67013PurposeExpresses malE-ELP[V-60] in E. coliDepositorInsertmalE-ELP[V-60]
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)
Plasmid#199166Purposeused to express a neutral GFP variant in E. coliDepositorInsertGFP(0)
UseTagsMGHHHHHHGGAExpressionMutationE16R, I138R, D207R, N222K, L246RPromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSG22-pBAD-TetR
Plasmid#102451PurposeExpresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.DepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Violet
Plasmid#160447PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Violet
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKFSS1_2
Plasmid#118227PurposeEmpty E. coli - B. burgdorferi shuttle vector; streptomycin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_2
Plasmid#118225PurposeEmpty E. coli - B. burgdorferi shuttle vector, gentamicin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG28-J23151-AraC
Plasmid#102453PurposeExpresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.DepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pet28a-His-tag-BS2
Plasmid#220347PurposeE. coli expression vector to purify BS2DepositorInsertBS2
UseTagsHis tagExpressionBacterialMutationPromoterAvailable SinceJune 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB1C3-J23110-B0034-mRFP1E
Plasmid#160442PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Salmonella MTAN
Plasmid#64041PurposeExpression of S. enterica MTAN in E. coliDepositorInsertMethylthioadenosine nucleosidase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOGG005
Plasmid#113980PurposeLevel 1 high copy number vector for E. coli expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-Ferritin-Dronpa
Plasmid#101194PurposeExpresses a Ferritin-Dronpa fusion construct in E. coliDepositorInsertFerritin-Dronpa
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationPromoterT7Available SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MK1274
Plasmid#71431PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…PromoterAvailable SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUMV19
Plasmid#67161Purposeinducible operation of the mevalonate pathway in E. coliDepositorInsertthe mevalonate pathway genes
UseTagsExpressionBacterialMutationPromoterlacZAvailable SinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 mouse PARG (439-959)
Plasmid#132615Purposeexpresses mouse PARG(439-959) in E. coli.DepositorInsertPARG (439-959)
UseTagsExpressionBacterialMutationPromoterAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.26-SS-352
Plasmid#68791PurposeZF9 Op x6 -- GFP-IRES-NTRDepositorInsertsZF9 Op 6x
EGFP
Nitroreductase
UseTagsIRESExpressionMammalianMutationPromoterAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CFP
Plasmid#173074PurposeExpression vector for E. coli producing target protein with N-terminal CFP fusionDepositorTypeEmpty backboneUseTagsmCeruleanExpressionBacterialMutationPromoterAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+6)
Plasmid#199160Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+6)
UseTagsMGHHHHHHGGAExpressionMutationE16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b_SNAP-ecDHFR
Plasmid#187108PurposeExpression of SNAP-ecDHFR fusion in bacteriaDepositorInsertSNAP-ecDHFR
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LolT_C41S
Plasmid#211789PurposeExpression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41SDepositorInsertlolt mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7 promoterAvailable SinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_2
Plasmid#118226PurposeEmpty E. coli - B. burgdorferi shuttle vector; kanamycin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Magenta
Plasmid#160446PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Magenta
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Yellow
Plasmid#160443PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Yellow
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
vgCam-pRSETb
Plasmid#193362PurposeA FRET-based calcium probe using SumireDepositorInsertvgCam
UseE.coli expressionTagsHis X6 tag and Strep tagExpressionMutationPromoterT7Available SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
A0A075HNX4
Plasmid#177695Purposeexpresses a fatty alcohol oxidase in E. coliDepositorInsertLong chain alcohol oxidase
UseTags10xHisExpressionBacterialMutationPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0248
Plasmid#162313PurposeReporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiTDepositorInsertT7prom-RBS-sfGFP-HiBiT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-hsGFP
Plasmid#64911PurposeEncodes a Fluorescent Reporter for Hydrogen Sulfide in E. ColiDepositorInserthsGFP
UseTagsHis TagExpressionBacterialMutationChromophore tyrosine of the protein mutated to TA…PromoteraraBADAvailable SinceMay 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAllet
Plasmid#202560PurposeE. coli cloning vector with large MCS (50+ unique restriction sites)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-mCherry
Plasmid#71737PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertmCherry
UseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterpRplsWTAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_CoumRS
Plasmid#64882PurposeExpresses Coumarinyl- tRNA synthetaseDepositorInsertCoum_RS
UseTagsFlagExpressionMutationPromoterCMVAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Pink
Plasmid#160445PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Pink chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Pink
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmN
Plasmid#221348PurposeExpress ChmN in E. coliDepositorInsertchmN
UseTagsHis6ExpressionBacterialMutationPromoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL13-MgtC bb118
Plasmid#158982PurposeConstitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.DepositorInsertmgtC
UseTagsExpressionBacterialMutationPromoterBba_J23118 (sigma 70)Available SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV-hEIF5B-1~1220(FL)
Plasmid#208636Purposefor E. coli expression of human eIF5B (ENST00000289371)DepositorInserthuman eIF5B (ENST00000289371) (EIF5B Human)
UseTags6xhisExpressionBacterialMutationContain SNP rs1376443, protein sequence not chang…PromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherryS131C
Plasmid#48732PurposeExpresses mCherryS131C in E. coli cellsDepositorInsertmCherryS131C
UseTags6xHisExpressionBacterialMutationchanged serine 131 to cysteinePromoterT5Available SinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Orange
Plasmid#160444PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Orange
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable SinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-35-H-6
Plasmid#67008PurposeExpresses malE-35-H-6 in E. coliDepositorInsertmalE-35-H-6
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0245
Plasmid#162284PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0729
Plasmid#162316PurposeTEV protease plasmid for T7-driven E. coli cell-free protein synthesisDepositorInsertT7prom-RBS-NET-TEVprotease
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD4 galK-GT
Plasmid#19183DepositorInsertgalK (galK E.coli)
UseRecombineeringTags50 nucleotides from N-term TAP, C-term TAP, and F…ExpressionMutationPromoterAvailable SinceJan. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SznE
Plasmid#139282Purposeexpresses SznE in E. coliDepositorInsertsznE
UseTagsExpressionBacterialMutationPromoterT7Available SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PAN
Plasmid#200971PurposeExpress poly-histidine tagged Methanococcus jannaschii PAN in E. coli recombinantlyDepositorInsertPAN
UseTags6xHis and 6xHis-thrombin siteExpressionBacterialMutationPromoterAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hdNadV
Plasmid#83362PurposeExpresses nadV from Haemophilus ducreyi in Escherichia coliDepositorInsertnadV
UseTagsnoneExpressionBacterialMutationPromoterT7Available SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only