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pCXLE-SOX2-17-p2a-KLF4

Plasmid

#193290

Purpose

episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion

Depositor

Hans Schöler

Publication

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pErCas12a EZ Clone

Plasmid

#132641

Purpose

Expresses ErCas12a and an sgRNA to target a region of interest

Depositor

Stephen Ekker

Publication

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

AAV and CRISPR

Tags

3x HA and SV40 NLS

Expression

Mammalian

Mutation

Q926R (Please see depositor comments) and BsaI si…

Promoter

CMV Promoter

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-PTEN

Plasmid

#116780

Purpose

Lentiviral expression of PTEN

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330S-6

Plasmid

#58782

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEERM1 Km

Plasmid

#64024

Purpose

Plasmid for integration into psbA2 site of Synechocystis 6803

Depositor

Pia Lindberg

Publication

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PpsbA2

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-CAG-iSeroSnFR-Nlgn

Plasmid

#128485

Purpose

Fluorescent reporter for serotonin (dendrite localization tag)

Depositor

Lin Tian

Publication

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Has service

AAV9

Insert

iSeroSnFR

Use

AAV

Tags

Full-length neuroligin, Ig-kappa leader, and cpsf…

Expression

Mammalian

Promoter

CAG

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Publication

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p416 25Q GAL

Plasmid

#1185

Depositor

Susan Lindquist

Publication

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-CMV-Flag-PGC1a-6His

Plasmid

#67637

Purpose

AAV vector expressing PGC1a gene

Depositor

Connie Cepko

Publication

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

PGC1a (Ppargc1a Mouse)

Use

AAV

Tags

6XHis and Flag tag

Promoter

CMV

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Publication

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVL-4

Plasmid

#104582

Purpose

Contains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

Depositor

Daniel Christ

Publication

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human CL kappa

Expression

Mammalian

Promoter

CMV

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mPapaya-pBAD

Plasmid

#54838

Purpose

Localization: Protein Expression Vector , Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Publication

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

6xHis and mPapaya

Expression

Bacterial

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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JDS70

Plasmid

#32285

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Type

Empty backbone

Use

T7

Tags

3X Flag and FOKI WT

Expression

Mammalian

Promoter

CMV

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pmCherry-Akt1(WT)

Plasmid

#86631

Purpose

Expresses mCherry-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCI H2B-RFP

Plasmid

#92398

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

IRES H2B RFP

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pT7-EGFP-C1-HsNot3

Plasmid

#37372

Depositor

Elisa Izaurralde

Publication

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot3 (CNOT3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HHM-iATPSnFR1.0

Plasmid

#102546

Purpose

expresses ATP sensor in bacteria

Depositor

Baljit Khakh

Publication

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Expression

Bacterial

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-AAVS1

Plasmid

#227272

Purpose

Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting AAVS1 (AAVS1 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-Neq2X7

Plasmid

#182366

Purpose

Expresses Neq2X7 DNA polymerase

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-myrTagRFP-T-P2A-post-eGRASP

Plasmid

#111590

Purpose

An AAV vector that expresses myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrTagRFP-T-P2A-post-eGRASP

Use

AAV

Promoter

tetO

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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