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We narrowed to 18,759 results for: nls

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Showing: 2261 - 2280 of 18759 results

  1. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100902-plasmid-map-sequence-id-195323

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  6. M-ST1n-VP64

    Plasmid
    #48675
    Purpose
    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VP64, nuclease-null
    Use
    CRISPR
    Tags
    NLS and VP64
    Mutation
    nuclease-null (D9A D598A H599A N622A)
    Promoter
    CMV
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48675-plasmid-map-sequence-id-71850

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  19. pUAS-V5-TurboID-NES

    Plasmid
    #116904
    Purpose
    Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.
    Depositor
    Norbert Perrimon
    Publication
    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboID
    Use
    Drosophila expression
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    116904-plasmid-map-sequence-id-224242

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  20. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    John Doench, David Root
    Publication
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    96921-plasmid-map-sequence-id-181715

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Showing: 2261 - 2280 of 18759 results