We narrowed to 11,909 results for: pcDNA

1. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

   Plasmid

   #175534

   Purpose

   Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag7-myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-sACE2-WT(732)-8h

   Plasmid

   #154101

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 (8his tag)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Short GSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 AKT3 HA (CT-gfp)

   Plasmid

   #27293

   Depositor

   Morris Birnbaum

   Insert

   protein kinase B gamma (Akt3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y473F

   Promoter

   Available since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA4.TO-ORF64-2xNSTREP

   Plasmid

   #136224

   Purpose

   Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF64

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TO-Nsp13_GC3opt

   Plasmid

   #157714

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3-Flag-hNF2

   Plasmid

   #107150

   Purpose

   mammalian expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

   Insert

   neurofibromin 2 (NF2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA6.2-YFP-CWT

   Plasmid

   #176689

   Purpose

   Fluorescent reporter for phagosomal escape of Staphylococcus aureus

   Depositor

   Martin Fraunholz

   Article

   Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

   Insert

   cell wall-targeting domain of Lysostaphin
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   deletion of protase domain of Lysostaphin

   Promoter

   CMV

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-SypHluorin 2x (S2x)

   Plasmid

   #37004

   Depositor

   Yongling Zhu, Stephen Heinemann

   Article

   Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

   Insert

   SypHluorin 2x (Syp Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Two copies of pHluorin inserted at site 184-185

   Promoter

   CMV

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-P25C-GFP

   Plasmid

   #1343

   Depositor

   Li-Huei Tsai

   Insert

   P25 (CDK5R1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-PalmPalm-ClopHensor

    Plasmid

    #25940

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    PalmPalm-ClopHensor
    

    Use

    Tags

    Gap20 and Strep-II

    Expression

    Mammalian

    Mutation

    N-term (20AA) of Gap-43

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA HA FAK Y407F

    Plasmid

    #50504

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y407F

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-ALK7 T194D

    Plasmid

    #80886

    Purpose

    mammalian expression of ALK7 T194D

    Depositor

    Aristidis Moustakas

    Article

    Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

    Insert

    ALK7 (Acvr1c Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T194D (constitutively active)

    Promoter

    CMV

    Available since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1 3xFlag IFIT2

    Plasmid

    #53555

    Purpose

    mammalian expression of IFIT2

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT2 (IFIT2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA FLAG TORC2

    Plasmid

    #22975

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

    Insert

    TORC2 (CRTC2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-GFP-p48

    Plasmid

    #11616

    Depositor

    Steven Johnson

    Insert

    Interferon-Stimulated Gene Factor 3 (IRF9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-myc-ARF

    Plasmid

    #19930

    Depositor

    Yue Xiong

    Article

    Zhang et al Cell. 1998 Mar 20. 92(6):725-34.

    Insert

    cyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-N-HA-NEK7 K64M

    Plasmid

    #75143

    Purpose

    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lys 64 mutated to Met; kinase inactive and F236L …

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(-) FLIPQTV3.0 100micro

    Plasmid

    #63730

    Purpose

    FLIPQTV3.0 glutamine sensor with D157N mutation

    Depositor

    Sakiko Okumoto

    Article

    Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

    Insert

    FLIPQTV3.0 sensor, 100microM affinity
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D157N in glutamine binding domain

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-pri-let-7b

    Plasmid

    #51378

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    pri-let-7b (MIRLET7B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BACE2_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176942

    Purpose

    Mammalian expression vector encoding BACE2 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    BACE2 (BACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge