We narrowed to 5,304 results for: pcDNA3.1(+)

1. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

   Plasmid

   #49061

   Purpose

   Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …

   Promoter

   CMV

   Available Since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5065 pcDNA3.1/V5-His-TOPO hBrd4 (888-1362)

   Plasmid

   #14444

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 (888-1362) (BRD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   amino acids 888-1362

   Promoter

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1417 (No intron)

   Plasmid

   #69904

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F169L in ZKSCAN1

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. (369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)

   Plasmid

   #168190

   Purpose

   HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-nLag6-(EAAAK)4-OGA(544-706)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL

   Plasmid

   #73210

   Purpose

   Expresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

   Insert

   KozATG-myc-dL5-2XG4S-mCer3-SKL (MYC Human, Synthetic)
   

   Use

   Tags

   The FAP and mCerulean3 are fused with 2 copies of…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available Since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

   Plasmid

   #49069

   Purpose

   Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   C563S,C568S,C577S,C582S in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP G protein Gamma 3(A73L,L74I,L75S) in pcDNA3.1

   Plasmid

   #42190

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

   Insert

   G protein Gamma 3(A73L,L74I,L75S) (Gng3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   A73L,L74I,L75S

   Promoter

   CMV

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-iRFP-P2A-Post-SPHERE-sfmTurquoise2-iGluSnFR

   Plasmid

   #187096

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Post-SPHERE-sfmTurquoise2-iGluSnFR
   

   Use

   Tags

   iRFP670-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

   Plasmid

   #225588

   Purpose

   Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-F8.2(S54L+T127A)-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)

    Plasmid

    #227620

    Purpose

    Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    R257A,R347A

    Promoter

    CMV, T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A/S1346A

    Plasmid

    #215902

    Purpose

    Expression of TNRC6A-CIM1 domain with S1332A/S1346A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528, S1332A/S1346A

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP

    Plasmid

    #225490

    Purpose

    Neuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    Neuronal expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217478

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1s
    

    Use

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217479

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1b
    

    Use

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS

    Plasmid

    #194751

    Purpose

    To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-MS2-HA-DmRoquin-del725-755-del790-812_AE

    Plasmid

    #148608

    Purpose

    Mammalian Expression of DmRoquin-del725-755-del790-812

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin-del725-755-del790-812 (roq Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-Rncp-iGluSnFR1

    Plasmid

    #187093

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-Rncp-iGluSnFR1
    

    Use

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-iRFP-P2A-Post-SPHERE-Rncp-iGluSnFR1

    Plasmid

    #187094

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-Rncp-iGluSnFR1
    

    Use

    Tags

    iRFP670-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-mCherry-P2A-Pre-SPHERE-sfmTurquoise2-iGluSnFR

    Plasmid

    #187095

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-sfmTurquoise2-iGluSnFR
    

    Use

    Tags

    mCherry-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1461R

    Plasmid

    #164002

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E1461R

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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