We narrowed to 7,506 results for: PAC

1. pSD068F

   Plasmid

   #218137

   Purpose

   Lentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD36-eGFP-IRES2-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSD113A

   Plasmid

   #218133

   Purpose

   For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD56
   

   Use

   Tags

   rpoZ

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJM154

   Plasmid

   #218121

   Purpose

   Hybrid circuit displaying FKBP12 bait and driving expression of luxAB

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   FKBP12
   luxAB
   

   Use

   Luciferase

   Tags

   434cI_RR69

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRhaCAST

   Plasmid

   #211791

   Purpose

   pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter

   Depositor

   Silvia Cardona

   Article

   Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Rhamnose-inducible

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-SpCas9HF1-P2A-puro

   Plasmid

   #214357

   Purpose

   Expresses SpCas9HF1 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9HF1 and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA4.TO_ORF18_TurboID_3xHA

   Plasmid

   #200017

   Purpose

   Expresses KSHV protein ORF18 fused at the C-terminal to TurboID-3xHA

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF18
   

   Use

   Tags

   TurboID-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA4.TO_ORF30_TurboID_3xHA

   Plasmid

   #200018

   Purpose

   Expresses KSHV protein ORF30 fused at the C-terminal to TurboID-3xHA

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF30
   

   Use

   Tags

   TurboID-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA4.TO_ORF31_TurboID_3xHA

   Plasmid

   #200019

   Purpose

   Expresses KSHV protein ORF31 fused at the C-terminal to TurboID-3xHA

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF31
   

   Use

   Tags

   TurboID-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA4.TO_ORF66_TurboID_3xHA

   Plasmid

   #200020

   Purpose

   Expresses KSHV protein ORF66 fused at the C-terminal to TurboID-3xHA

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF66
   

   Use

   Tags

   TurboID-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA4.TO_3xHA_TurboID_ORF34

    Plasmid

    #200021

    Purpose

    Expresses KSHV protein ORF34 fused at the N-terminal to 3xHA-TurboID

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF34
    

    Use

    Tags

    3xHA-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM1-zeo G226A ORF29

    Plasmid

    #200024

    Purpose

    Lentiviral vector for expression of KSHV ORF29 with G226A mutation

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF29
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glycine 226 changed to alanine

    Promoter

    CMV

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-FKBP-PIP4K2A-Kina

    Plasmid

    #202745

    Purpose

    expresses recruitable fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mCherry:FKBP1A(3-108) : [GGSA]4GG :PIP4K2A (33-406) (PIP4K2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OsMTI-1B Oryza sativa Metallothionein 1B

    Plasmid

    #200295

    Purpose

    Bacterial expression plasmids for production of recombinant Oryza sativa Metallothionein 1B

    Depositor

    Artur Krężel

    Article

    Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

    Insert

    Gene of metallothionein with Chiting Binding Domanin-Intein (LOC4332487 Oryza sativa)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197958

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCVp and pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC1300_pUB10_pco-vCASphi_E9t_V2_U6_AtPDS3_gRNA10

    Plasmid

    #197963

    Purpose

    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-vCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 and pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-N1-AAGAB

    Plasmid

    #198333

    Purpose

    Expresses AAGAB-GFP fusion in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AAGAB (AAGAB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOPO812

    Plasmid

    #195306

    Purpose

    pBAD322-MycaIDCas-ΔCas6-RGR-lacZs, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked lacZ spacer 5.

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    McCAST Cascade Δcas6 and ribozyme flanked lacZ spacer 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose inducible arapBAD

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGO186N-KS1

    Plasmid

    #174301

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDGO186N-KS2

    Plasmid

    #174302

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSF-p15A-T7- YFP

    Plasmid

    #186568

    Purpose

    p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSFDuet-1_FGF21

    Plasmid

    #182406

    Purpose

    Bacterial expression of fgf21

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    fgf21 (FGF21 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSFDuet-1_VNp-LZ_CydAB-mNeongreen

    Plasmid

    #182393

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ
    CydAB-mNeongreen
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJH4140

    Plasmid

    #191479

    Purpose

    Pacr-2s-loxP::EBFP::loxP::Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Chrimson
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2s

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJH3650

    Plasmid

    #191446

    Purpose

    Pacr-2s-Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression of Chrimson RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Chrimson
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2s

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJH2684

    Plasmid

    #192061

    Purpose

    Pacr-5-TeTx::wCherry unc-54 3' UTR C.elegans B-MN and other neurons expression of TeTx RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Tetanus Toxin Light Chain
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pacr-5

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJH4211

    Plasmid

    #191532

    Purpose

    Pacr-2s-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans A/B MN expression of GtACR2 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GtACR2
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2s

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDGO186N-KS3

    Plasmid

    #174303

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag

    Plasmid

    #186708

    Purpose

    N-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

    Depositor

    Paula Tamagnini

    Article

    Mascia et al Green Chemistry, 24 (2022):6156

    Insert

    alr4766
    

    Use

    Synthetic Biology

    Tags

    His-Tag (6His)

    Expression

    Mutation

    Promoter

    PpsbA2*

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

    Plasmid

    #186710

    Purpose

    N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

    Depositor

    Paula Tamagnini

    Article

    Mascia et al Green Chemistry, 24 (2022):6156

    Insert

    alr4766
    

    Use

    Synthetic Biology

    Tags

    His-Tag (6His)

    Expression

    Mutation

    Promoter

    Ptrc.x.tetO2

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJH2842

    Plasmid

    #179540

    Purpose

    Pacr-5::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans B-MN and other neurals expression of miniSOG-SL2 RFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pacr-5

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSIN-Tpi1

    Plasmid

    #175940

    Purpose

    knockout mouse Tpi1

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Tpi1 gRNA (Tpi1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSIN-Olfr2

    Plasmid

    #175935

    Purpose

    knockout mouse Olfr2

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Olfr2 gRNA (Or6a2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-CVm-Sec22b

    Plasmid

    #168029

    Purpose

    Expresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-Sec22b(A131-H190)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-CVm-VAMP8

    Plasmid

    #168028

    Purpose

    Expresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP8(G7-R67)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUE1-TSP-ORF68 R14A/K310A

    Plasmid

    #162655

    Purpose

    Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids R14 and K310 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLJM1-zeo-2xStrep-ORF68 C52A

    Plasmid

    #162660

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C52 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUE1-TSP-ORF68 K435A

    Plasmid

    #162651

    Purpose

    Expresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acid K435 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUE1-TSP-ORF68 K450A/R451A

    Plasmid

    #162653

    Purpose

    Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K450 and R451 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

    Plasmid

    #162639

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids K174, R179, and K182 mutated to alani…

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKH-1699

    Plasmid

    #124227

    Purpose

    Mammalian SpCas9-HF1 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge