We narrowed to 8,206 results for: Dos
-
Plasmid#231115PurposeFragmid fragment: (guide cassette) ABE activity-based selection cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only
-
AA498
Plasmid#231119PurposeFragmid fragment: (guide cassette) ABE activity-based selection CD274 positive controlDepositorInsertsgCD274 + ABE splice-targeting sgRNA
UseCRISPRExpressionBacterialAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA505
Plasmid#231120PurposeFragmid fragment: (guide cassette) CBE activity-based selection CD274 positive controlDepositorInsertsgCD274 + CBE splice-targeting sgRNA
UseCRISPRExpressionBacterialAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA568
Plasmid#231117PurposeFragmid fragment: (guide cassette) CBE activity-based selection cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA509
Plasmid#231116PurposeFragmid fragment: (2A Selection) ABE activity-based selection intronDepositorInsertPuroN'-intron-PuroC'
UseCRISPRExpressionBacterialAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)
Plasmid#226884PurposeExpresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cellsDepositorInsertNaVAb
TagsMBPExpressionInsectMutationR4A, N49K, L109A, M116VPromoterPolyhedrinAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-KDAC8 H143A
Plasmid#224342PurposeExpresses human KDAC8 (HDAC8) H143A in E. coliDepositorInsertKDAC8 H143A (HDAC8 Human)
TagsTEV-cleavable His6ExpressionBacterialMutationHistidine 143 mutated to alaninePromoterT5Available SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacI-KDAC7 CD
Plasmid#224345PurposeExpresses human KDAC7 (HDAC7) catalytic domain in insect cellsDepositorInsertKDAC7 (HDAC7 Human)
TagsTEV-cleavable His6ExpressionInsectMutationOnly includes residues 521-942 (catalytic domain).PromoterPolyhedrinAvailable SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
300_pETcon_SARS2_FLip
Plasmid#222230Purposeyeast surface display of the SARS-CoV-2 FLip variant RBDDepositorAvailable SinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEXP(FLAG/HA-NSP1-K164-H165A)
Plasmid#188782PurposeExpresses FLAG/HA-NSP1-K164-H165A in mammalian cells (SARS-CoV-2 mutant NSP1)DepositorInsertFLAG/HA-NSP1-K164-H165A (SARS-CoV-2)
UseFrt site to insert into hek293 t-rex genomeTagsFLAG/HAExpressionMammalianMutationChanged Lysine 164 to Alanine and Histidine 165 t…PromoterCMV Dox inducibleAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
RT-4
Plasmid#220443PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.DepositorAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SCML2_3
Plasmid#220878PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV DJ-1 C106A
Plasmid#220914PurposeExpresses DJ-1 with the C106A mutationDepositorAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV hSyn mRuby
Plasmid#220912PurposeNeuronal specific expression of mRubyDepositorInsertmRuby
UseAAVExpressionMammalianPromoterhSynAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPI470
Plasmid#207436PurposeInducible extrachromosomal overexpression vector - mNeonGreen - C-terminalDepositorInsertC-terminal mNeonGreen
UseUnspecifiedAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPI450
Plasmid#207433PurposeInducible extrachromosomal overexpression vector - mNeonGreen - N-terminalDepositorInsertN-terminal mNeonGreen
UseUnspecifiedAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPI465
Plasmid#207435PurposeREMI integrated Expression for mNeonGreen Fusions N-terminalDepositorInsertN-terminal mNeonGreen
UseUnspecifiedAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPI471
Plasmid#207437PurposeREMI integrated Expression for mNeonGreen Fusions C-terminalDepositorInsertC-terminal mNeonGreen
UseUnspecifiedAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EFNB2 (D62Q)
Plasmid#200976PurposeMammalian expression plasmid for myc-tagged EFNB2 (D62Q specificity mutant)DepositorInsertEFNB2 (EFNB2 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationD62Q (increases specificity towards henipaviral G…PromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2084
Plasmid#137726PurposeGateway vector for inducible stem loop RNAi in T.b. brucei 2T1DepositorTypeEmpty backboneUseRNAi; Trypanosoma brucei tet inducible stem loopAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST tol2 UbB polyA
Plasmid#188701PurposeGateway destination vector containing the zebrafish ubiquitin B polyadenylation sequenceDepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianAvailable SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML104-natR412
Plasmid#162042PurposeExpresses Cas9 and contains natR412 guide which targets the natR geneDepositorInsertnatR412 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML107-natR854
Plasmid#162043PurposeExpresses Cas9 and contains natR854 guide which targets the natR geneDepositorInsertnatR854 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH1498
Plasmid#178600PurposePrgef-1 INS-18::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-18 fused to GFPDepositorAvailable SinceJan. 5, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-anticalin (E60amb)-His
Plasmid#168043PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationGlu 60 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E143amb)-His
Plasmid#168046PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationGlu 143 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (S87amb)-His
Plasmid#168044PurposeExpresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationSer 87 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N116amb)-His
Plasmid#168045PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationAsn 116 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N21amb)-His
Plasmid#168041PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationAsn 21 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-FLN-ELP-His-ybbr
Plasmid#168047PurposeExpresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, ddFLN4, and ybbr tagExpressionBacterialPromoterT7 promoterAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (I55amb)-His
Plasmid#168042PurposeExpresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationIle 55 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG422_UV5
Plasmid#165607PurposeVector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1332K and R1333G mutations in SpCas9PromoterlacUV5 driving Cas9 KG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOCT4-Cyan-pac
Plasmid#160871PurposeMarmoset TargetingDepositorInsertmCerulean3
UseMarmoset targetingAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-15
Plasmid#160715PurposeFor C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic 3X-FLAG peptide
UseSynthetic BiologyAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PDCD6IP_BRO1
Plasmid#109994PurposeProtein expression and purification of PDCD6IP_BRO1DepositorInsertPDCD6IP_BRO1 (PDCD6IP Human)
ExpressionBacterialAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOK6_IRS
Plasmid#109886PurposeProtein expression and purification of DOK6_IRSDepositorInsertDOK6_IRS (DOK6 Human)
ExpressionBacterialAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBR5_PABP
Plasmid#109877PurposeProtein expression and purification of UBR5_PABPDepositorInsertUBR5_PABP (UBR5 Human)
ExpressionBacterialAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOK3_IRS
Plasmid#109885PurposeProtein expression and purification of DOK3_IRSDepositorInsertDOK3_IRS (DOK3 Human)
ExpressionBacterialAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER-CAmut
Plasmid#155237Purposefor mammalian overexpression of the mutated ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) (Usp19 Rat)
Tags6XHis-3XFlagExpressionMammalianMutationCys(545) to AlaPromoterCMVAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TERF1_TRF
Plasmid#110040PurposeProtein expression and purification of TERF1_TRFDepositorInsertTERF1_TRF (TERF1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_WDR5_WD40
Plasmid#110046PurposeProtein expression and purification of WDR5_WD40DepositorInsertWDR5_WD40 (WDR5 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP2_SH3
Plasmid#110016PurposeProtein expression and purification of GRAP2_SH3DepositorInsertGRAP2_SH3 (GRAP2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DLG1_PDZ_1
Plasmid#109998PurposeProtein expression and purification of DLG1_PDZ_1DepositorInsertDLG1_PDZ_1 (DLG1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DLG1_PDZ_2
Plasmid#109999PurposeProtein expression and purification of DLG1_PDZ_2DepositorInsertDLG1_PDZ_2 (DLG1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DLG2_PDZ_2
Plasmid#110000PurposeProtein expression and purification of DLG2_PDZ_2DepositorInsertDLG2_PDZ_2 (DLG2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DLG2_PDZ_3
Plasmid#110001PurposeProtein expression and purification of DLG2_PDZ_3DepositorInsertDLG2_PDZ_3
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DLG4_PDZ_2
Plasmid#110002PurposeProtein expression and purification of DLG4_PDZ_2DepositorInsertDLG4_PDZ_2 (DLG4 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DLG4_PDZ_3
Plasmid#110003PurposeProtein expression and purification of DLG4_PDZ_3DepositorInsertDLG4_PDZ_3 (DLG4 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TLN2_IRS
Plasmid#109939PurposeProtein expression and purification of TLN2_IRSDepositorInsertTLN2_IRS
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only