We narrowed to 22,370 results for: ESC;

1. pMyosin-miRFP670nano

   Plasmid

   #127431

   Purpose

   LC-Myosin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano-LC-Myosin
   

   Tags

   miRFP670nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pREF-GFP kan

   Plasmid

   #122061

   Purpose

   reporter plasmid for termination and terminator override to express GFP. Marked with URA3

   Depositor

   Daniel Reines

   Article

   Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

   Inserts

   intergenic terminator from yeast IMD2
   green fluorescent protein
   

   Expression

   Yeast

   Promoter

   GAL1 and GAL1 comes in pYES2

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046871

   Plasmid

   #145724

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGBW-m4046731

   Plasmid

   #145633

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp2

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. LbCas12a

   Plasmid

   #188493

   Purpose

   Expresses FLAG tagged LbCas12a

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12a
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBW-m4046833

   Plasmid

   #145649

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp9

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. DGK beta

   Plasmid

   #35405

   Depositor

   Stephen Prescott, Robert Lefkowitz

   Article

   Nelson et al Science. 2007 Feb 2;315(5812):663-6.

   Insert

   DGK beta (DGKB Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

   Plasmid

   #159896

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR
   

   Expression

   Worm

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nano-lantern-mitochondria/pcDNA3

   Plasmid

   #51973

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Available Since

   March 28, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pTriEx4-H6-FGB

    Plasmid

    #49203

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    H6-FGB
    

    Tags

    His tag + Other tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    None

    Promoter

    CMV

    Available Since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. DGK epsilon

    Plasmid

    #35407

    Depositor

    Stephen Prescott, Robert Lefkowitz

    Article

    Nelson et al Science. 2007 Feb 2;315(5812):663-6.

    Insert

    DGK epsilon (DGKE Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pZyxin-miRFP703

    Plasmid

    #79999

    Purpose

    Zyxin labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    Zyxin (ZYX Human)
    

    Tags

    miRFP703

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-NHERF1-PDZ1iP

    Plasmid

    #28295

    Depositor

    Maria-Magdalena Georgescu

    Article

    Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

    Insert

    NHERF1 (NHERF1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains aa 1-148 (deletion of PDZ2 and EB domain…

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pQLinkHD_EBFP2-LSSmOrange

    Plasmid

    #118856

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    EBFP2-LSSmOrange
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMB1-spect-PthrC-eGFP

    Plasmid

    #107408

    Purpose

    This plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Expression

    Bacterial

    Promoter

    PthrC

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXGFPC-5 Pxyl-dcas9 (Spa)

    Plasmid

    #133318

    Purpose

    expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus pasteurianus)
    

    Use

    Unspecified

    Promoter

    xylose

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pActinin-miRFP670nano

    Plasmid

    #127432

    Purpose

    Alpha actinin labeling with near-infrared fluorescent protein

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    Alpha-actinin-miRFP670nano
    

    Tags

    miRFP670nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Nano-lantern(Ca2+)_300/pcDNA3

    Plasmid

    #51983

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. P303-FBP_2

    Plasmid

    #139693

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22-HT/prsA

    Plasmid

    #73624

    Purpose

    Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

    Depositor

    T. J. Kappock, James Williamson

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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